癫痫清颗粒对海人藻酸致癫痫大鼠海马组织中IL-6含量及GFAP、Iba-1表达的影响  

Effects of Dianxianqing Granule on the IL-6 Content and GFAP,Iba-1 Expressions in Hippocampus Tissue of Rats with Kainate-induced Epilepsy

作　　者：齐越[1] 李纪彤[2] 姜鸿[1] 王光函[1] 刘小虎[1] 向绍杰[1] 秦文艳[1] 韦丹[3] 朱竟赫 贾冬[3] 

机构地区：[1]辽宁省中医药研究院药理室,沈阳110034 [2]辽宁中医药大学附属第三医院综合病房,沈阳110032 [3]辽宁中医药大学省部共建中医脏象理论及应用教育部重点实验室,沈阳110847

出　　处：《中国药房》2017年第28期3927-3931,共5页China Pharmacy

基　　金：辽宁省博士科研启动基金资助项目(No.201601385)

摘　　要：目的:研究癫痫清颗粒对海人藻酸致癫痫大鼠海马组织中白细胞介素6(IL-6)含量及胶质纤维酸性蛋白(GFAP)、离子钙接头分子1(Iba-1)表达的影响,探讨该药防治癫痫的作用机制。方法:将大鼠随机分为假手术组(蒸馏水)、模型组(蒸馏水)、苯妥英钠组(0.03 g/kg,阳性对照)和癫痫清颗粒低、中、高剂量组(4.74、9.47、18.94 g/kg,以生药计),每组20只,每天ig给药1次,连续7d。末次给药1 h后,除假手术组外的其余各组大鼠均于左侧海马CA1区单次注射海人藻酸复制癫痫模型,观察大鼠行为学变化及死亡情况。造模24 h后,采用酶联免疫吸附法检测大鼠海马组织中IL-6含量,采用尼氏染色法对海马组织神经元进行计数,采用免疫组化法检测大鼠海马组织中GFAP、Iba-1表达。结果:与假手术组比较,模型组大鼠造模后发生明显癫痫症状,部分大鼠死亡;海马组织中IL-6含量及神经元数目明显减少(P<0.01),GFAP、Iba-1表达明显增强(P<0.01)。与模型组比较,各给药组大鼠癫痫症状及死亡情况得到改善,而海马组织中IL-6含量虽均不同程度升高,但差异无统计学意义(P>0.05);苯妥英钠组和癫痫清颗粒中、高剂量组大鼠海马组织神经元个数明显增加(P<0.01),GFAP表达明显降低(P<0.01);苯妥英钠组和癫痫清颗粒高剂量组大鼠海马组织中Iba-1表达明显降低(P<0.01)。结论:癫痫清颗粒可通过抑制海马组织中GFAP和Iba-1的表达、增加海马组织中神经元数量,从而发挥防治癫痫的作用。OBJECTIVE：To study the effects of Dianxianqing granule on the interleukin（IL-6）content and glial fibrillary acid-ic protein（GFAP）,ionized calcium linker molecules 1（Iba-1）expressions in hippocampus tissue of rats with kainate-induced epi-lepsy,and explore its mechanism of preventing and treating epilepsy. METHODS：Rats were randomly divided into sham operation group （distilled water）,model group （distilled water）,phenytoin group （0.03 g/kg,positive control） and Dianxianqing granule low-dose,medium-dose,high-dose groups（4.74,9.47,18.94 g/kg,calculated by crude drug）,20 in each group. Rats were intra-gastrically administrated once a day,for 7 d. After 1 h of last administration,except for sham operation group,rats in other groups received single injection of kainite in hippocampus CA1 of left side to induce the epilepsy model. Behavioral changes and death of rats were observed. After 24 h of modeling,enzyme-linked immunosorbent method was used to detect the IL-6 content in hippocam-pus tissue of rats,Nissl staining was used to count the hippocampus neurons,and immunohistochemistry was used to detect the GFAP,Iba-1 expressions in hippocampus tissue of rats. RESULTS：Compared with sham operation group,rats in model group had obvious epilepsy symptoms after modeling,and parts of rats died;IL-6 content and number of neurons in hippocampus tissue were obviously decreased （P〈0.01）, while GFAP, Iba-1 expressions were obviously enhanced （P〈0.01）. Compared with model group,epilepsy symptoms and death in each administration group had improved,while IL-6 content in hippocampus tissue were in-creased to varying degrees,with no statistical significance （P〉0.05）. The numbers of neurons in phenytoin group,Dianxianqinggranule medium-dose, high-dose groups were obviously in-creased（P〈0.01）;GFAP expression was obviously decreased （P〈0.01）. Iba-1 expressions in hippocampus tissue in phenyt-oin group,Dianxianqing granule high-dose group were obvi-ously decreased （P〈0.01

关 键 词：癫痫清颗粒 白细胞介素6 胶质纤维酸性蛋白 离子钙接头分子1 大鼠 

分 类 号：R285[医药卫生—中药学]