回药核心方油溶液对离体大鼠胸主动脉血管环的舒张作用及机制研究  

Study on the Diastolic Effect and Mechanism of Hui Medicine Hexin Oil Solution on Isolated Thoracic Aortic Vascular Rings of Rats

作　　者：张鹏[1] 郝银菊[2] 任俊[2] 韩亮娟 余建强[2] 张万年[2] 周茹[2,3,4,5] 

机构地区：[1]宁夏医科大学总医院药剂科,银川750004 [2]宁夏医科大学药学院,银川750004 [3]宁夏回药现代化工程技术研究中心,银川750004 [4]宁夏回医药协同创新中心,银川750004 [5]宁夏医科大学回医药现代化省部共建教育部重点实验室,银川750004

出　　处：《中国药房》2017年第28期3952-3955,共4页China Pharmacy

基　　金：国家科技支撑计划课题(No.2013BAI11B07);宁夏回族自治区科技攻关计划项目(No.2012);大学生创新/创业计划项目(No.201510752008)

摘　　要：目的:研究回药核心方油溶液对离体大鼠胸主动脉血管环的舒张作用及机制,为其用于心血管疾病的治疗提供参考。方法:取出大鼠胸主动脉血管环后浸泡于克氏营养液(K-H)中,以1×10^(-6)mol/L去甲肾上腺素(PE)或60 mmol/L氯化钾(KCl)致血管环收缩,采用生物信号采集分析系统测定0.020 4、0.040 8、0.061 2、0.081 6、0.102 0 mg/mL核心方油溶液对血管环的舒张作用,计算舒张率;分别以0.1 mmol/L一氧化氮合酶抑制剂左旋硝基精氨酸甲酯(L-NAME)、环氧合酶抑制剂吲哚美辛(INDO)、钾离子通道阻滞剂格列本脲(Gli)孵育血管环20 min后,测定上述5种质量浓度核心方油溶液对PE预收缩血管环的舒张作用,计算舒张率;试验均以K-H溶液为空白对照。结果:与空白对照比较,0.020 4~0.102 0 mg/mL核心方油溶液对PE、KCl预收缩血管环均有明显的舒张作用(P<0.05或P<0.01),且具有浓度依赖性。INDO预处理后可减弱核心方油溶液对PE预收缩血管环的舒张作用,舒张率较空白对照组差异无统计学意义(P>0.05);而Gli、L-NAME预处理不影响核心方油溶液对PE预收缩血管环的舒张作用,舒张率较空白对照组仍明显升高(P<0.05或P<0.01)。结论:核心方油溶液可呈浓度依赖性地舒张PE、KCl预收缩的胸主动脉血管环,其作用机制可能与激活环氧合酶途径有关。OBJECTIVE：To study the diastolic effect and mechanism of Hui medicine Hexin oil solution on isolated thoracic aortic vascular rings of rats,and provide reference for its treatment for cardiovascular diseases. METHODS：Thoracic aortic vascular rings of rats were taken and then soaked in Kelvin’s nutrient solution（K-H）. Using 1×10-6mol/L norepinephrine（PE）or 60mmol/L potassium chloride（KCl）for inducing the contraction of vascular rings,biological signal acquisition and analysis system was used to determine the diastolic effect and mechanism of Hexin oil solution with concentrations of 0.020 4,0.040 8,0.061 2,0.081 6,0.102 0 mg/mL on vascular rings,and diastolic rate was calculated. After culturing vascular rings by 0.1 mmol/L nitric oxide synthase inhibitor L-nitro-arginine methyl ester（L-NAME）,cyclooxygenase inhibitor indomethacin（INDO）,and potassium ion channel blocker glibenclamide（Gli）for 20 min,the diastolic effects of above-mentioned 5 mass concentrations of Hexin oil solution on the contraction of vascular rings pre-contracted by PE were determined,and diastolic rate was calculated. The test was based on K-H solution as blank control. RESULTS：Compared with blank control,Hexin oil solution with concentration of 0.020 4-0.102 0 mg/mL had obvious diastolic effect on the contraction of vascular rings induced by PE and KCl（P〈0.05 or P〈0.01）,showing concentration-dependent relationship. INDO pre-treatment can relieve the diastolic effect of Hexin oil solution on vascular rings pre-contracted by PE;and compared with blank control group,the diastolic rate had no statistical significance（P〉0.05）.While the pre-treatment of Gli,L-NAME did not affect the diastolic effect of Hexin oil solution on vascular rings pre-contracted by PE;and compared with blank control group,diastolic rate was obviously increased（P〈0.05 or P〈0.01）. CONCLUSIONS：Hexin oil solution can concentration-dependently conduct the relaxation of thoracic aortic vascular rings pre-contracted by PE,KCl

关 键 词：回药 核心方油溶液 离体胸主动脉血管环 血管舒张 环氧合酶抑制剂 钾离子通道阻滞剂 一氧化氮合酶抑制剂 

分 类 号：R285[医药卫生—中药学]