异氟醚吸入麻醉对大鼠海马脑源性神经营养因子与Bcl-2表达的影响  

Effects of isoflurane inhalation anesthesia on the expressions of BDNF and Bcl-2 in rat hippocampus

作　　者：张亦南[1,2] 朱宇航 张超[1,2] 刘德行 朱昭琼[1,2] 

机构地区：[1]遵义医学院附属医院麻醉科,贵州遵义563000 [2]贵州省麻醉与器官保护基础研究重点实验室,贵州遵义563000

出　　处：《重庆医学》2017年第29期4044-4046,4050,共4页Chongqing medicine

基　　金：贵州省科学技术基金资助项目(黔科合J字LKZ[2011]29)

摘　　要：目的观察异氟醚(ISO)吸入麻醉对大鼠海马脑源性神经营养因子(BDNF)及B细胞淋巴瘤/白血病-2(Bcl-2)分子表达的影响。方法将54只SD大鼠分为对照组(n=6)、氧气(O2)组(n=24)和ISO组(n=24),对照组直接处死留取标本,O2组给予吸入40%O2和空气混合气体1h,ISO组以3%ISO诱导麻醉并以1.8%ISO维持1h。分别于麻醉前、诱导后、麻醉后30min、麻醉后60min、苏醒即刻观察大鼠呼吸频率及经皮血氧饱和度(SpO2)。O2组和ISO组分别于处理后6、12、24、72h处死大鼠取海马,采用半定量反转录PCR(RT-PCR)检测海马BDNF mRNA、Bcl-2mRNA的表达。结果大鼠呼吸频率在诱导后及麻醉后30、60min较麻醉前均有不同程度降低(P<0.05);SpO2在诱导后、麻醉后30、60min及苏醒即刻与麻醉前比较,差异无统计学意义(P>0.05)。ISO组大鼠海马BDNF mRNA表达水平与O2组比较在麻醉后12、24h降低(P<0.05);麻醉后ISO组大鼠海马BDNF mRNA表达水平随时间呈下降趋势,在12、24h明显低于对照组(P<0.05)。ISO组大鼠海马Bcl-2mRNA表达水平在所有时间点均低于对照组与O2组(P<0.05)。结论 ISO麻醉可对大鼠海马BDNF及Bcl-2的表达产生抑制作用。Objective To observe the effects of isoflurane （ISO） inhale anesthesia on the expression of brain-derived neurotrophic factor （BDNF） and Bcl-2 of hippocampal in rats. Methods A total of 54 SD rats were divided into 3 groups., control group （n=6） ,O2 group （n=24） and ISO group （n=24）. All rats were given 1 week to adapt the environment. Then, rats in the control group were directly Sacrificed to collect hippocampi specimens; rats in the O2 group inhaled mixed gas containing 40% O2 and air for 1 hour; rats in the ISO group anesthetized with 3% ISO and maintained for 1 h with 1.8% ISO after righting reflex disappeared,inhaling 40% O2 in the whole process of anesthesia. Respiratory rate and percutaneous oxygen saturation （SpO2） of the rats were observed before anesthesia, after induction, 30 min after anesthesia, 60 min after anesthesia and at the moment of analepsia, respectively. Rats in the O2 group and ISO group were sacrificed to extract hippocampi specimens at 6,12,24 and 72 h after treatment, respectively. The BDNF and Bcl-2 mRNA expression levels in hippocampus of rats were detected using the semi-quantitative reverse transcription PCR （RT-PCR）. Results Compared to that before anesthesia, the respiratory rates after induction, 30 min after anesthesia and 60 min after anesthesia were decreased （P〈0. 05） ,while no apparent changes was found in the SpO2 （P〉0.05）. Compared to the O2 group,the relative expressions of BDNF mRNA in hippocampi of rats in the ISO group were obviously decreased at 12 and 24 h after the treatment （P〈0.05）. After anesthesia,the relative expression of BDNF mRNA in rat hippocampus in the ISO group was decreased as time goes on,and the expression levels at 12 and 24 h after the treatment were lower than those in the control group （P〈0.05）. Compared to the O2 group and controi group,the relative expression levels of Bcl-2 mRNA in hippoeampi of the rats in the ISO group were lower at all time points for detection （P〈0.05）. Conclusion

关 键 词：异氟醚 海马 脑源性神经营养因子 BCL-2 

分 类 号：R614[医药卫生—麻醉学]