南极冰藻DNA光修复酶脂质体的制备及其质量评价  

Preparation of DNA Photolyase Liposome from Antarctic Ice Microalgae and Its Quality Evaluation

作　　者：李然 陈璐[1,2] 齐娟[1] 金青 缪锦来[2,3,4] LI Ran CHEN Lu QI Juan JIN Qing MIAO Jin-lai(Qingdao University of Science and Technology, Qingdao 266042, China First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China Qingdao National Laboratory for Marine Science and Technology, Qingdao 266235, China Qingdao University, Qingdao 266071, China)

机构地区：[1]青岛科技大学,山东青岛266042 [2]国家海洋局第一海洋研究所,山东青岛266061 [3]青岛海洋科学与技术国家实验室,山东青岛266235 [4]青岛大学,山东青岛266071

出　　处：《化学与生物工程》2017年第10期25-31,共7页Chemistry & Bioengineering

基　　金：国家自然科学基金项目(41576187);中央级公益性科研院所基本科研业务费专项资金项目(2016Q10);国家自然科学基金委员会-山东省人民政府海洋科学研究中心联合资助项目(U1606403);国家海洋公益性行业科研专项(201405015);山东省自主创新及成果转化专项(2014ZZCX06202);青岛市创业创新领军人才计划项目(15-10-3-15-(44)-zch);山东省重点研发计划(2016YYSP017;2016ZDJS06A03;2017GHY15112)

摘　　要：以包封率为评价指标,氯仿-乙醚混合溶剂(2∶3,体积比)为有机相,固态大豆卵磷脂和胆固醇为膜材,采用逆向蒸发法制备南极冰藻DNA光修复酶脂质体。经单因素实验和正交实验确定光修复酶脂质体的最佳制备工艺条件为:膜材比3∶1、药脂比1∶10、油相与水相体积比4∶1、超声时间6min,在此条件下,光修复酶脂质体的平均包封率达到(44.13±2.90)%。所制得的光修复酶脂质体呈圆形或椭圆形,平均粒径为(490.9±2.3)nm,Zeta电位在-30^-60mV范围内,制剂的质量标准符合中国药典(2015版)要求,为DNA光修复酶在日用防晒品中的应用提供了可靠的理论依据。Using entrapment efficiency as an evaluation index, the mixed solvent of chloroform-ether（ volume ratio 2：3） as an organic phase,and solid soybean lecithin and cholesterol as membrane materials,we prepared DNA photolyase liposome from Antarctic ice microalgae by a reverse evaporation method. Through single-factor experiment and orthogonal experiment,we optimized preparation conditions as follows：the ratio of membrane to material was 3 ： 1,the ratio of drug to lipid was 1 ＂ 10,the volume ratio of oil phase to water phase was 4：1, and the ultrasonic time was 6 rain. Under above conditions, the average entrapment efficiency of photolyase lipo- some was （44.13±2.90）%. The prepared photolyase liposome was round or oval sphere,the average particle size was （490.9±2.3） nm,and Zeta potential was in the range of --30 mV to --60 mV. The quality standard of the preparation meets the requirements of Chinese Pharmacopoeia （2015 edition） ,which provides a reliable the- oretical basis for the application of DNA photolyase in the daily sun screen.

关 键 词：脂质体 包封率 南极冰藻DNA光修复酶脂质体 正交实验 质量评价 

分 类 号：TQ461[化学工程—制药化工]