5个树莓品种红外光谱差异性及其亲缘关系分析  

Difference of Infrared Spectra and Genetic Relationship on 5 Varieities of Raspberry

作　　者：李雕 杨静慧 杨仁杰 张伟玉 吴楠 张超 

机构地区：[1]天津农学院园艺园林学院,天津300384 [2]天津农学院工程技术学院,天津300384

出　　处：《天津农学院学报》2017年第3期24-27,31,共5页Journal of Tianjin Agricultural University

基　　金：天津市农业科技成果转化与推广项目"彩叶树优良品种繁殖;栽培技术示范与推广"(201502100);天津市科技成果转化及产业化推进计划项目"优质彩叶北美海棠新品种种苗的规模化繁育"(14ZXNZNC0040)

摘　　要：为比较不同树莓品种的差异,了解品种间的亲缘关系,本文通过红外光谱差谱峰、面域差、相关性分析和聚类分析等方法,对5个树莓品种的种子进行了差异性及其亲缘关系分析。结果表明:在普通红外光谱图中,5个树莓品种种子的红外光谱图相似。但是,通过其差减光谱发现,在3 332、2 932、1 740、1 650、1 437、1 150、711 cm-1附近都出现了差谱峰;吸光度差异最大的峰为2 920 cm-1、1 740~1 748 cm-1、1 036~1 052 cm-1附近,对应的官能团为淀粉类物质亚甲基C—H、油脂类成分酯羰基C=O、多糖类C—O;‘诺娃’与‘香妃’的面域差最大,亲缘关系最远,‘红宝’与‘诺娃’的面域差最小,亲缘关系最近;各树莓品种的红外光谱曲线的相关性较高,相关系数为0.934~0.993。聚类分析将5个树莓品种分为4类,其中‘红宝’与‘诺娃’为同一类,与面域差分析结果一致。In order to compare the difference and to explore the genetic relationship of raspberry varieties, the difference of infrared spectra and genetic relationship on 5 varieties of raspberry was researched by the way of FTIR differential spectra peak, area deviation of spectrum, correlation analysis and hierarchical clustering. The results showed that the infrared spectra of five varieties were similar in the first grade. But the differential peaks were appeared in near 3 332, 2 932, 1 740, 1 650, 1 437, 1 150 and 711cm1 on the basis of the subtraction spectra. The absorbance were most different in near 2 920 cm1, 1 740- 1 748 cm^-1, 1 036-1 052 cm^-1, corresponding to the functional group of farinaceous substance （the methylene C--H）, oil （ester carbonyl group C = O）, polysaccharide （C-O）. The genetic relationship of ＇Nova＇ and ＇XF＇ was the farthest due to their largest area difference, while the genetic relationship of ＇HB＇ and ＇Nova＇ was the closest due to their smallest area difference among all varieties. There was the higher correlation between the infrared spectrum curves of any two raspberry varieties based on 0.934~ 0.993 correlation coefficient. The five raspberry varieties were divided into 4 categories by hierarchical clustering, in which ‘HB＇ and ‘NW＇ were in the same categories. The result was same as area difference analysis.

关 键 词：树莓 品种 红外光谱 差谱 亲缘关系 

分 类 号：S663.2[农业科学—果树学]