普通小麦Glu-3位点基因单元型的分离和序列分析  

Isolation and Sequence Analysis of LMW-GS Gene at the Glu-3 Loci in Common Wheat

作　　者：董雪 王增 刘梦 杨燕 韩冰 李淑芬 赵献林[2] 

机构地区：[1]内蒙古农业大学生命科学学院植物生物技术功能实验室,内蒙古呼和浩特010018 [2]河南省农业科学院小麦研究所/河南省小麦生物学重点实验室/小麦国家工程实验室/农业部黄淮中部小麦生物学与遗传育种重点实验室,河南郑州450002

出　　处：《麦类作物学报》2017年第10期1276-1284,共9页Journal of Triticeae Crops

基　　金：国家自然科学基金项目(31271726);内蒙古农业大学优秀青年科学基金项目(2014XYQ-18);内蒙古自治区自然科学基金项目(2014MS0338);内蒙古自治区产业创新创业人才团队项目(201503004)

摘　　要：为了从分子水平上探讨优质小麦资源中LMW-GS等位基因与小麦品质的关系,以及在改善小麦品质方面的潜在价值,利用小麦Glu-A3和Glu-B3基因的特异引物从强筋型、中筋型和弱筋型小麦共计10份材料中分离出LMW-GS基因后进行序列分析。结果表明,共发现14个新的核苷酸变异类型和4个肽链变异类型。其中,14个新的核苷酸变异类型中,4个为Glu-A3基因变异类型,1个为Glu-B3基因变异类型,9个为Glu-D3基因变异类型。值得注意的是,有2个半胱氨酸数目特殊的亚基类型被发现,一个是来自师栾02-1含有9个半胱氨酸残基的GluA3-18基因,另一个是来自偃展4110含有7个半胱氨酸残基的GluD3-13基因。Low-molecular-weight glutenin subunits （LMW-GS） are major fractions of wheat gluten,and have great effects on dough extensibility and food processing quality. In this study,the specific primers of Glu-A3 and Glu-B3 genes were used to identify and amplify the allelic variations of LMW-GS genes from ten wheat varieties with different gluten content. A total of 14 new DNA haplotypes were identified,including four haplotypes at Glu-A3,one haplotype at Glu-B3 and nine haplotypes at Glu-D3. Among them,the corresponding peptide of GluA3-18 from wheat variety Shiluan 02-1 contains nine cysteine residues,and the corresponding peptide of GluD3-13 from Yanzhan 4110 has seven cysteine residues,both of which belong to the specific subunits compared with others with eight cysteine residues. The results provide the basis for further study of LMW-GS genes and quality improvement of wheat.

关 键 词：普通小麦 低分子量麦谷蛋白亚基 基因分离 Glu-3位点 

分 类 号：S512.1[农业科学—作物学] S330