生物素化微细纤维肽修饰IGF-1对心肌梗死兔肌球蛋白基因表达的影响  

Influence of biotinylated microfibrinopeptide modified IGF-1 on myosin gene expression in rabbits with myocardial infarction

作　　者：胡云华[1] 张明[1] 梅志亮[1] 肖祖克[1] 

机构地区：[1]江西省人民医院二部心内科一区,江西南昌330006

出　　处：《中国当代医药》2017年第28期33-35,共3页China Modern Medicine

摘　　要：目的研究生物素化微细纤维肽修饰IGF-1对心肌梗死兔肌球蛋白基因表达的影响。方法将28只健康的雄性新西兰白兔随机分为对照组、实验组1、实验组2、实验组3,每组7只。对照组开胸但不进行冠状动脉左前降支结扎,实验组1、2、3建立兔心肌梗死模型。建模成功后,实验组1注射生物素化微细纤维肽修饰的IGF-1,实验组2注射未修饰的IGF-1,对照组(未结扎)与实验组3(心肌梗死)注射同等剂量的生理盐水。术后8周,测定心肌肌球蛋白基因表达。结果与对照组比较,实验组3心肌肌球蛋白基因的表达水平显著降低,实验组1、2心肌肌球蛋白基因的表达水平显著提高(P<0.05),但实验组1的心肌肌球蛋白基因表达水平又显著高于实验组2(P<0.05)。结论急性心肌梗死会减弱兔心肌肌球蛋白的基因表达,IGF-1和生物素化微细纤维肽修饰IGF-1均能改善心肌梗死兔肌球蛋白的表达,但经过生物素化微细纤维肽修饰的IGF-1比单纯IGF-1的效果更优。Objective To study the influence of biotinylated microfibrinopeptide modified IGF-1 on myosin gene expres- sion in rabbits with myocardial infarction.Methods A total of 28 healthy male New Zealand white rabbits were random- ly divided into the control group,experimental group 1,experimental group 2,and experimental group 3,and there were 7 ones in each group.In the control group,rabbits were performed with thoracotomy but without ligation of left anterior de- scending branch of coronary artery.For experimental group 1,2,and 3,model of myocardial infarction in rabbits was es- tablished.After successful modeling,biotinylated microfibrinopeptide modified IGF-1 was injected in the experimental group 1.In the experimental group 2,biotinylated microfibrinopeptide IGF-1 without modification was injected.In the experimental group 3 （myocardial infarction） and control group （without ligation）,the same dose of normal saline was in- jected.The gene expression of myosin was determined 8 weeks after surgery.Results Compared with the control group, theexpression level of myosingene in the experimental group 3 was significantly decreased,but in the experimental group 1 and 2,the gene expression level were greatly increased （P〈0.05）.The expression level of myosin gene in the ex- perimental group 1 was significantly higher than that in the experimental group 2.Conclusion Acute myocardial infarc- tion will attenuate the gene expression of myosin in rabitts.Both IGF-1 and biotinylated microfibrinopeptide modified IGF-1 can improve gene expression of myosin in rabbits with myocardial infarction,but the effect is better in the latter.

关 键 词：生物素化微细纤维肽修饰IGF-1 心肌梗死 肌球蛋白 基因表达 

分 类 号：R332[医药卫生—人体生理学]