盐负荷和碱负荷对孕晚期大鼠离体子宫收缩力和催产素受体水平的影响  

Effects of Salt and Alkali Load on Isolated Uterine Contractility and Oxytocin Receptor Level in Rats During Late Pregnancy

作　　者：胡梦双 李巨[2] 

机构地区：[1]锦州医科大学中国人民解放军第202医院研究生培养基地,沈阳110003 [2]中国人民解放军第202医院妇产科,沈阳110003

出　　处：《中国医科大学学报》2017年第10期891-894,共4页Journal of China Medical University

基　　金：辽宁省科学技术计划(2014225005)

摘　　要：目的探讨盐负荷和碱负荷对孕晚期大鼠离体子宫收缩力和催产素受体(OXTR)水平的影响。方法将42只孕晚期大鼠随机均分为3组,A组饮用自来水,B组饮用2%Na Cl溶液,C组饮用5%Na HCO3溶液;分别于0、12、24、36、48、60和72 h时从3组中各随机选取2只大鼠麻醉,先剖腹采腹主动脉血行动脉血气分析,再将子宫纵向剖取数个均等肌条,检测其肌张力,最后采用ELISA法测定肌条中OXTR的含量。结果 3组在0 h时血Na+、HCO3-浓度差异均无统计学意义(P>0.05);在其余各时间点,B组和C组血Na+浓度均显著高于A组(P<0.05),C组血HCO3-浓度显著高于A组和B组(P<0.05)。3组在0 h时子宫肌条收缩的振幅和频率差异均无统计学意义(P>0.05);B组在其余各时间点的振幅和频率(除72 h振幅外)均显著高于A组(P<0.05);C组在12、24和60 h时振幅显著高于A组(P<0.05),在48、60和72 h时频率显著高于A组(P<0.05)。3组0 h时OXTR含量差异均无统计学意义(P>0.05);其余各时间点,B组OXTR含量显著高于A组(P<0.05);C组在12、24、60和72 h时OXTR含量显著高于A组(P<0.05)。结论盐负荷可使子宫收缩振幅、频率及子宫表面OXTR表达显著增强;碱负荷60 h时可使子宫收缩振幅、频率及OXTR表达三者均显著增强。Objective To investigate changes in uterine contractility and oxytocin receptor （OXTR） level after salt and alkali load in rats during late pregnancy. Methods A total of 42 pregnant rats were randomly divided into groups given tap water （group A）, 2% NaCl solution （group B）, or 5% NaHCO3 solution （group C）. Two rats were taken from each of the three groups at 0, 12,24,36,48,60, and 72 h, respectively, for arterial blood gas analysis and preparation of uterine muscle strips for muscle tension measurement. The OXTR levels were measured using ELISA. Results There were no significant differences in serum sodium and bicarbonate ion concentrations, muscle tension, or OXTR levels among the groups at 0 h （P 〉 0.05）. At other time points, the sodium ion concentration in group B and group C was significantly higher than in group A （P 〈 0.05）, and bicarbonate ion concentration in group C was higher than in group A and group B （P 〈 0.05）. Muscle tension （including amplitude and frequency） in group B was significantly greater than in group A （except at 72 h） （P 〈 0.05）. The amplitude in group C was higher than in group A at 12,24,and 60 h （P 〈 0.05）,while the frequency was higher than in group A at 48, 60,and 72 h （P 〈 0.05）. The OXTR level in group B was significantly higher than in group A （P 〈 0.05）,and was higher in group C than in group A at 12,24,60, and 72 h （P 〈 0.05）. Conclusion Salt and alkali loading can increase uterine contractility and OXTR levels at 60 h.

关 键 词：盐负荷 碱负荷 子宫 催产素受体 肌张力 ELISA 

分 类 号：R714.46[医药卫生—妇产科学]