毛细管电泳法测定复合维生素B片含量  

Determination of Compound Vitamin B Tablets by Capillary Electrophoresis

作　　者：严健[1] 王小如[1] 张君倩[2] 叶飞云[2] 曾海虹[2] 

机构地区：[1]厦门大学化学系,厦门361005 [2]厦门市药品检验所,厦门361012

出　　处：《中国现代应用药学》2002年第4期326-327,共2页Chinese Journal of Modern Applied Pharmacy

摘　　要：建立了毛细管电泳测定复合维生素B片中维生素B1、维生素B2、烟酰胺含量的方法。方法 :缓冲液为 0 .0 2mmol/L硼酸盐溶液 ,毛细管电泳柱为 5 0 μm× 5 0cm ,检测波长为 2 0 0nm。 结果 :回收率为 98.7%～ 10 1.0 % ,相对标准偏差为 1.8%～ 3.2 %。结论 :本方法简便、快速。OBJECTIVE: To establish a method for the simultaneous determination of vitamin B1, vitamin B2 and nicotinamide in compound vitamin B tablets by capillary electrophoresis. METHOD: The electrophoretic medium was composed of 0. 02mol/L borate solution. The uncoated capillary used was 50μm×50cm. The detection wanelength was at 200nm. RESULTS: The recoveries ranged from 98.7% to 101.0% with relative standard deviations from 1.8%～3.5%. CONCLUSION: This method was simple, rapid and reliable.

关 键 词：含量 毛细管电泳 复合维生素B片 维生素B1 维生素B2 烟酰胺 

分 类 号：R977.22[医药卫生—药品]