丰富环境对抑郁大鼠行为学及海马组织中丝裂原活化蛋白激酶磷酸酶-1表达的影响  

Effects of enriched environment on behavior and expression of mitogen-activated protein kinase phosphatase-1 in hippocampus of depression rats

作　　者：赵利芹[1] 李晏[2] 邵秋静 吕婷婷[1] 谷景阳 刘聪[1] 韩金红[3] 张向阳[4] 王长虹[1] 

机构地区：[1]新乡医学院第二临床学院,河南新乡453002 [2]郑州大学公共卫生学院,河南郑州450001 [3]新乡医学院基础医学院组织与胚胎学教研室,河南新乡453003 [4]北京回龙观医院生物精神病研究中心,北京100096

出　　处：《新乡医学院学报》2017年第9期798-802,共5页Journal of Xinxiang Medical University

基　　金：国家自然科学基金资助项目(编号:81671346);河南省自然科学基金资助项目(编号:300410223);2015年研究生科技创新支持计划资助项目(编号:CX201548Y);新乡医学院科研培育基金资助(编号:3ZD117)

摘　　要：目的探讨丰富环境(EE)对慢性不可预见性轻度应激(CUS)抑郁模型大鼠行为学及海马组织中丝裂原活化蛋白激酶磷酸酶-1(MKP-1)表达的影响,为抑郁症的治疗提供线索。方法 40只Sprague-Dawley大鼠随机分为对照组、CUS组、氟西汀组及EE组,每组10只。CUS组、氟西汀组、EE组大鼠分别给予8周的CUS,从第5周起EE组及氟西汀组大鼠分别给予EE干预及氟西汀灌胃4周;对照组大鼠正常环境饲养8周。采用体质量增加量、旷场试验、蔗糖偏好等评估各组大鼠行为学的变化,采用免疫印迹法检测大鼠海马组织中MKP-1蛋白的表达。结果各组大鼠造模前体质量、水平运动距离、直立次数、穿格次数、蔗糖偏好指数比较差异均无统计学意义(P>0.05)。与对照组比较,造模后CUS组、氟西汀组及EE组大鼠体质量增加减少,水平运动距离、直立次数、穿格次数减少,蔗糖偏好指数降低(P<0.05);CUS组、氟西汀组及EE组大鼠体质量增加、水平运动距离、直立次数、穿格次数及蔗糖偏好指数两两比较差异无统计学意义(P>0.05)。氟西汀及EE干预后,CUS组大鼠体质量增加、水平运动距离、直立次数、穿格次数、糖水偏好指数与对照组大鼠比较均减少(P<0.05);氟西汀组及EE组大鼠体质量增加、水平运动距离、直立次数、穿格次数及蔗糖偏好指数与对照组比较差异均无统计学意义(P>0.05);氟西汀组和EE组大鼠体质量增加、水平运动距离、直立次数、穿格次数及蔗糖偏好指数较CUS组均升高(P<0.05);EE组大鼠体质量增加、水平运动距离、直立次数、穿格次数及蔗糖偏好指数与氟西汀组比较差异均无统计学意义(P>0.05)。与对照组比较,CUS组和EE组大鼠海马组织中MKP-1蛋白表达显著升高(P<0.05);氟西汀组大鼠海马组织中MKP-1蛋白表达与对照组比较差异无统计学意义(P>0.05)。与CUS组比较,氟西汀组大鼠海马组织中MKP-1蛋白表达明显�Objective To investigate the effect of enriched environment （EE） on behavior and expression of mitogenactivated protein kinase phosphatase-1 （MKP-1） in hippocampus of depression rats induced by chronic unpredicted mild stress （CUS） and to provide clues for the molecular mechanism of treating depression.Methods Forty Sprague-Dawley rats were randomly divided into control group,CUS group,fluoxetine group and EE group,with 10 rats in each group.The rats in CUS group,fluoxetine group and EE group were given 8 weeks of CUS,and from the fifth week,the rats in EE group and fluoxetine group were given EE and fluoxetine for 4 weeks,respectively.The changes of behavioristic of the rats in the four groups were evaluated by body mass gain,open field test,and sucrose preference.The expression of MKP-1 in hippocampus was detected by Western blot.Results There was no significant difference in body mass,distance of horizontal movement,the number of upright,the times of passing through the grid and sucrose preference index among the four groups（P 〉 0.05）.After modeling,compared with the control group,the body mass gain,distance of horizontal movement,the number of up-right,the times of passing through the grid and sucrose preference index in the CUS group,fluoxetine group and EE group were decreased significantly（P 〈 0.05）;there was no significant difference in the body mass gain,distance of horizontal movement,the number of up-right,the times of passing through the grid and sucrose preference index among the CUS group,fluoxetine group and EE group（P 〉 0.05）.After intervening by fluoxetine and EE,the body mass gain,distance of horizontal movement,the number of up-right,the times of passing through the grid and sucrose preference index in the CUS group were lower than those in the control group（P 〈0.05）;but there was no significant difference in the body mass gain,distance of horizontal movement,the number of up-right,the times of passing through the grid and sucrose preference index between the

关 键 词：抑郁症 丝裂原活化蛋白激酶磷酸酶-1 丰富环境 氟西汀 

分 类 号：G822.02[文化科学—体育训练]