1840MHz电磁辐射对雄性SD大鼠性激素的影响及其抗氧化损伤作用研究  

Effects of 1840 MHz Radiation-frequency Electromagnetic Radiation on the Sex Hormone Secretion of Male SD Rats and Its Anti-oxidative Damage Effect

作　　者：袁梦 林艳云[2] 郭奇彦 高鹏[2] 苗霞[2] 郎海洋[2] 张锦鹏[3] 袁堉琨 刘军叶[2] 曾丽华[2] 

机构地区：[1]第四军医大学军事预防医学系,陕西西安710032 [2]第四军医大学军事预防医学系放射医学教研室,陕西西安710032 [3]第四军医大学,陕西西安710032

出　　处：《现代生物医学进展》2017年第28期5406-5411,共6页Progress in Modern Biomedicine

基　　金：国家重点基础研究发展计划(973计划)(2011CB503704);国家自然科学基金项目(51677189,51437008);陕西省自然科学基金项目(2015JM8454)

摘　　要：目的:探讨1840MHz电磁辐射对雄性SD大鼠性激素分泌的影响及其抗氧化损伤作用。方法:将48只成年雄性SD大鼠按体质量随机分为8组,即2组假辐照组和6组辐照组,辐照组给予频率为1840MHz,比吸收率(SAR)分别为0.2、0.5、1.5 W/kg的电磁辐射,连续辐照1周或3周(5 d/周,30 min/d)。辐照后立即麻醉SD大鼠并进行心脏采血,分离血清,采用放射免疫法测量血清中卵泡刺激素(FSH)、生长激素(GH)、促性腺激素释放激素(Gn RH)、黄体生成素(LH)、睾酮(T)的浓度。睾丸组织称重并给予多聚甲醛固定,石蜡包埋切片后采用苏木精-伊红(HE)染色,用显微镜观察睾丸组织形态结构,采用免疫组织化学法观测睾丸组织内热休克蛋白70(HSP70)和DNA氧化损伤的标志物8-OHd G的分布及表达情况。结果:与对照组相比,辐照组大鼠睾丸组织结构无显著损伤,但是经比吸收率为0.2 W/Kg和0.5 W/Kg的电磁辐射辐照3周(5 d/周,30 min/d)后,辐照组FSH,Gn RH,LH水平降低,差异具有统计学意义(p<0.05),睾丸组织中HSP70和8-OHd G的表达无明显改变。结论:1840MHz电磁辐射对雄性SD大鼠睾丸的形态结构无明显影响,而睾丸的部分生殖内分泌功能改变,没有引起大鼠睾丸组织明显的氧化应激损伤。Objective： To investigate the effects of 1840 MHz radiation-frequency electromagnetic radiation on the sex hormone secretion and anti-oxidative damage of male SD rats. Methods： 48 SD rats were divided into eight groups：two sham exposure groups （control group） and six exposure groups. The exposure groups were exposed to 1840MHz, with SAR （Specific absorption rate） of 0.2, 0.5, 1.5 W/kg, for 3 or 5 weeks of 5 d/week and 30 min/d. The SD rats were anesthetized and put to execution after exposure immediately. Testis and blood of heart were collected and the testis was fixed by paraformaldehyde. Hematoxylin-eosin （HE） staining combined with microscope to observe the morphology of left testicular tissue. The right testis were stained in immunocytochemistry to observe the distribution of heat shock protein 70 （HSP70） and 8-OHdG which is a kind of DNA oxidative damage marker. Radioimmunoassay was used to detect in the concentration of FSH, GH, GnRH, LH and Testosterone. Results： Compared with the control group, themorphology and the distribution of HSP70 and 8-OHdG in testicular tissue didn＇t have any extremely difference in every exposure group. But after radiation of 1840MHz, with SAR of 0.2, 0.5 W/kg for 3 weeks of 5 d/week and 30 min/d, the levels of FSH, GnRH and LH in the irradiated group were lower than those in control group and the difference was statistically significant （p 0.05）. Conclusions： After the exposure of 1840 MHz radiation, the morphology change of testis didn＇t induced, but the reproductive endocrine function of testis changed a lot to some content, while it couldn＇t induce the oxidative damage of testis.

关 键 词：1840MHz电磁辐射 SD大鼠 睾丸 抗氧化损伤 

分 类 号：Q64[生物学—生物物理学] Q492.4