第二代高通量测序技术用于进口细菌型微生物肥料菌群分析  

Next Generation Sequencing Technique for the Analysis of the Bacterial Community Structure about Bacterial Microbial Fertilizers

作　　者：刘玉莉[1] 魏霜[2] 刘碧琳[1] 鄞杰平[1] 林利平[1] 

机构地区：[1]汕头出入境检验检疫局,广东汕头515041 [2]广东出入境检验检疫局检验检疫技术中心,广东广州510000

出　　处：《安徽农业科学》2017年第28期146-147,150,共3页Journal of Anhui Agricultural Sciences

基　　金：国家质量监督检验检疫总局科技计划项目(2015IK078)

摘　　要：[目的]分析细菌型微生物肥料菌群结构。[方法]采用第二代高通量测序技术对细菌型微生物肥料菌群结构进行分析。[结果]产品中测序出的111个OUT具体分属于24个属的细菌,其中共有21个OUT鉴定到种;植物乳杆菌(Lactobacillus plantarum)和短乳杆菌(Lactobacillus brevis)占菌群的主要位置,分别是96.29%和0.66%,其他菌占3.05%;该样品中含有假单胞菌属(Pseudomonas sp.),这提示样品中可能含有植物病原细菌。[结论]将第二代高通量测序技术用于微生物肥料菌群分析,为进出口微生物肥料产品的快速分析提供了一种方法。[Objective]To study the bacterial community structure of import microbial fertilizers.[Method]Next generation sequencing technique was used to analyze the bacterial community structure of import microbial fertilizers.[ Result] 111 OUTs were identified as 24 genus.Lactobacil-lus plantarum and Lactobacillus brevis accounted for 96.29% and 0.66%, and the other bacteria accounted for 3.05%.Pseudomonas sp.has been found which means plant pathogenic bacteria may contain in this product.[Conclusion]Next generation sequencing technique has been de-veloped for analyzing the bacterial community structure of microbial fertilizers, which can be used for the import or export of microbial fertilizers.

关 键 词：第二代高通量测序 微生物肥料 菌群 

分 类 号：S144[农业科学—肥料学]