UPLC同时测定补肾强身片中6种化学成分的含量  

Simultaneous Determination of Six Components in Bushen Qiangshen Pills

作　　者：林秀莲 宋粉云 李华[3] 陈宇轩 周颖仪[3] 

机构地区：[1]广东药科大学中药学院,广州510006 [2]广东药科大学药学院,广州510006 [3]广东省药品检验所,广州510018

出　　处：《中国实验方剂学杂志》2017年第20期56-60,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：上海市药物(中药)代谢产物研究重点实验室开放课题(2015SHDX1001)

摘　　要：目的:采用超高效液相色谱法(UPLC)同时测定补肾强身片中原儿茶酸、绿原酸、金丝桃苷、特女贞苷、朝藿定C和淫羊藿苷的含量。方法:以Agilent SB-C18色谱柱(4.6 mm×100 mm,2.7μm),乙腈-0.1%磷酸水溶液为流动相进行梯度洗脱,流速0.5 m L·min^(-1),柱温40℃,检测波长260,325,360,224,270 nm。结果:6个成分的检测范围分别为原儿茶酸0.78~201.00 mg·L^(-1)(r=0.999 9),绿原酸0.79~202.60 mg·L^(-1)(r=0.999 7),金丝桃苷0.93~238.39 mg·L^(-1)(r=0.999 9),特女贞苷0.66~169.62 mg·L^(-1)(r=0.999 9),朝藿定C 0.81~206.85 mg·L^(-1)(r=0.999 9),淫羊藿苷0.74~190.85 mg·L^(-1)(r=0.999 9)。平均回收率分别为97.6%(RSD 1.0%),99.6%(RSD 1.5%),99.7%(RSD 1.5%),97.2%(RSD 0.8%),95.5%(RSD 0.4%),96.3%(RSD 0.9%)。结论:该方法简便可靠,可用于补肾强身片的质量控制。Objective： To simultaneously determinate the contents of protocatechuic acid,chlorogenic acid,hyperoside,specnuezhenide,epmedin C and icariin in Bushen Qiangshen pills（ BQP） by developing an Ultra high performance liquid method（ UPLC）. Method： The UPLC separation was performed on an Agilent SBC18 reversed-phase column（ 4. 6 mm × 100 mm,2. 7 μm） with acetonitrile-0. 1% phosphoric acid solution as the mobile phase for gradient elution. The detective wavelength was set at 260,325,360,224,270 nm. Result： The calibration curve was linear when amount was within 0. 78-201. 00 mg·L^（-1） for protocatechuic acid（ r = 0. 999 9）;0. 79-202. 60 mg·L^（-1） for chlorogenic acid（ r = 0. 999 7）; 0. 93-238. 39 mg·L^（-1） for hyperoside（ r = 0. 999 9）;0. 66^（-1）69. 62 mg·L^（-1） for specnuezhenide（ r = 0. 999 9）; 0. 81-206. 85 mg·L^（-1） for epmedin C（ r = 0. 999 9）;and 0. 74^（-1）90. 85 mg·L^（-1） for icariin（ r = 0. 999 9）. The recoveries were 97. 6%,99. 6%,99. 7%,97. 2%,95. 5%,96. 3% with RSDs of 1. 0%,1. 5%,1. 5%,0. 8%,0. 4% and 0. 9%,respectively. Conclusion： This simple and reliable method can be used for the quality control of BQP.

关 键 词：补肾强身片 原儿茶酸 绿原酸 金丝桃苷 特女贞苷 朝藿定C 淫羊藿苷 

分 类 号：R284.1[医药卫生—中药学]