不同引经药配伍大黄-丹参药对抗大鼠肝纤维化的比较  

Effect of Herb Pair of Rhei Radix et Rhizoma and Salviae Miltiorrhizae Radix et Rhizoma with Different Medicinal Guides on Hepatic Fibrosis Rats

作　　者：宋献美 石科 葛文静 王慧森[2] 贾士奇[2] 傅蔓华[2] 梁瑞峰[2] 

机构地区：[1]河南医学高等专科学校,郑州451191 [2]河南省中医药研究院,郑州450004

出　　处：《中国实验方剂学杂志》2017年第20期132-137,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河南省科技攻关计划项目(162102310373);河南省中医药科学研究专项(2015ZY2072)

摘　　要：目的:观察不同引经药配伍大黄-丹参药对抗大鼠肝纤维化的效应差异,探讨引经药的增效作用。方法:健康雄性SD大鼠60只,随机分为正常组,模型组,丹参-大黄药对组(5.0 g·kg^(-1)),药对加桔梗组(5.0 g·kg^(-1)+1.5 g·kg^(-1)),药对加柴胡组(5.0 g·kg^(-1)+1.5 g·kg^(-1))和药对加细辛组(5.0 g·kg^(-1)+0.3 g·kg^(-1)),除正常组外,其余各组均腹腔注射四氯化碳(CCl4)复制肝纤维化模型。从第3周开始,除注射造模外,各实验组分别给予相应药物灌胃,正常组、模型组灌胃双蒸水,连续6周。末次给药后,取血和肝脏,检测血清中丙氨酸氨基转移酶(ALT),天门冬氨酸氨基转移酶(AST),碱性磷酸酶(ALP)的活性;酶联免疫吸附法(ELISA)测定血清中层黏连蛋白(LN),透明质酸(HA)和Ⅲ型前胶原(PCⅢ)的含量;酸水解法检测肝匀浆中羟脯胺酸(HYP)的含量;观察肝脏的组织形态改变;免疫组化法检测肝组织中转化生长因子-β1(TGF-β1)的表达。结果:与模型组比较,各给药组大鼠血清中ALT,AST,ALP活性和LN,HA,PCⅢ的含量显著降低,肝匀浆中HYP含量降低,肝组织TGF-β1表达减少(P<0.05,P<0.01),肝组织结构明显改善;与药对组相比,药对加柴胡组大鼠ALT,AST,ALP活性和LN,HA,PCⅢ的含量降低,肝组织TGF-β1表达减少(P<0.05,P<0.01)。结论:大黄-丹参药对能有效减轻肝纤维化的程度,引经药柴胡可增强大黄-丹参药对的抗肝纤维化作用。Objective： To compare the effect of herb pair of Rhei Radix et Rhizoma and Salviae Miltiorrhizae Radix et Rhizoma with different medicinal guides on hepatic fibrosis rats. Method： Sixty SD rats were randomly divided into normal group,model group,herb pair group（ 5. 0 g·kg^（-1））,herb pair plus Platycodonis Radix（ 5. 0 g·kg^（-1）＋ 1. 5 g·kg^（-1））,herb pair plus Bupleuri Radix group（ 5. 0 g·kg^（-1）＋ 1. 5 g·kg^（-1）） and herb pair plus Asari Radix et Rhizom group（ 5. 0 g·kg^（-1）＋ 0. 3 g·kg^（-1））. The hepatic fibrosis rat models were induced through intraperitoneal injection with CCl4. Since the 3 rdweek after modeling,different drugs were administrated by gavage for 6 weeks. Rats were put to death after the last administration,and their serum was collected to measure activities of serum alanin aminotransferase（ ALT）,aspartate aminotransferase（ AST） and alkaline phosphatase（ ALP）. Levels of laminin（ LN）,hyaluronic acid（ HA） and procollagen Ⅲ（ PCⅢ） in serum were detected by enzyme linked immunosorbent assay（ ELISA）. The content of hydroxyproline（ HYP） in liver homogenate was measured by acid hydrolysis method. The expression of transforming growth factor-β1（ TGF-β1） in liver was analyzed by immunohistochemical method. Result： Compared with the model group,the levels of ALT,AST,ALP,LN,HA,PCⅢ and the content of HYP in experimental groups were significantly decreased,the expression of TGF-β1 in liver was reduced（ P〈0. 05,P〈0. 01）,the liver pathological fibrosis was obviously relieved.Compared with the herb pair group,the levels of ALT,AST,ALP,LN,HA,PCⅢ and the expression of TGF-β1 in the herb pair plus Bupleuri Radix group were significantly decreased（ P〈0. 05,P〈0. 01）. Conclusion： The herb pair of Rhei Radix et Rhizoma and Salviae Miltiorrhizae Radix et Rhizoma has a protective effect on liver fibrosis rat,and Bupleuri Radix could further improve the curative effect.

关 键 词：大黄-丹参 肝纤维化 引经药 柴胡 转化生长因子-β1(TGF-β1) 

分 类 号：R285.5[医药卫生—中药学]