Conditional and unconditional QTLs mapping of gluten strength in common wheat (Triticum aestivum L.)  

作　　者：LIU Tong-tong LIU Kai WANG Fang-fang ZHANG Ying LI Qing-fang ZHANG Kai-ran XIE Chu-peng TIAN Ji-chun CHEN Jian-sheng 

机构地区：[1]State Key Laboratory of Crop Biology/Group of Wheat Quality Breeding, Key Laboratory of Crop Water Physiology and Drought-tolerance Germplasm Improvement, Ministry of Agriculture/College of Agronomy, Shandong Agricultural University, Tai'an 271018, P. R. China

出　　处：《Journal of Integrative Agriculture》2017年第10期2145-2155,共11页农业科学学报（英文版）

基　　金：support from the Natural Science Foundation of Shandong Province,China (ZR2015CM036);the Molecular Foundation of Main Crop Quality,the Ministry of Science and Technology of China (2016YFD0100500);the Project of Science and Technology of Shandong “Wheat Breeding by Molecular Design”,China (2016LZGC023);the Research Fund for Agricultural Big Data Project,China

摘　　要：Dissecting the genetic relationships among gluten-related traits is important for high quality wheat breeding. Quantita- tive trait loci （QTLs） analysis for gluten strength, as measured by sedimentation volume （SV） and gluten index （GI）, was performed using the QTLNetwork 2.0 software. Recombinant inbred lines （RILs） derived from the winter wheat varieties Shannong 01-35xGaocheng 9411 were used for the study. A total of seven additive QTLs for gluten strength were identi- fied using an unconditional analysis. QGi1D-13 and QSv1D-14 were detected through unconditional and conditional QTLs mapping, which explained 9.15-45.08% of the phenotypic variation. QTLs only identified under conditional QTL mapping were located in three marker intervals： WPT-3743-GLU-D1 （1D）, WPT-7001-WMC258 （1B）, and WPT-8682-WPT-5562 （1B）. Six pairs of epistatic QTLs distributed nine chromosomes were identified. Of these, two main effect QTLs （QGi1D-13 and QSvlD-14） and 12 pairs of epistatic QTLs were involved in interactions with the environment. The results indicated that chromosomes 1B and 1D are important for the improvement of gluten strength in common wheat. The combination of conditional and unconditional QTLs mapping could be useful for a better understanding of the interdependence of different traits at the QTL molecular level.Dissecting the genetic relationships among gluten-related traits is important for high quality wheat breeding. Quantita- tive trait loci （QTLs） analysis for gluten strength, as measured by sedimentation volume （SV） and gluten index （GI）, was performed using the QTLNetwork 2.0 software. Recombinant inbred lines （RILs） derived from the winter wheat varieties Shannong 01-35xGaocheng 9411 were used for the study. A total of seven additive QTLs for gluten strength were identi- fied using an unconditional analysis. QGi1D-13 and QSv1D-14 were detected through unconditional and conditional QTLs mapping, which explained 9.15-45.08% of the phenotypic variation. QTLs only identified under conditional QTL mapping were located in three marker intervals： WPT-3743-GLU-D1 （1D）, WPT-7001-WMC258 （1B）, and WPT-8682-WPT-5562 （1B）. Six pairs of epistatic QTLs distributed nine chromosomes were identified. Of these, two main effect QTLs （QGi1D-13 and QSvlD-14） and 12 pairs of epistatic QTLs were involved in interactions with the environment. The results indicated that chromosomes 1B and 1D are important for the improvement of gluten strength in common wheat. The combination of conditional and unconditional QTLs mapping could be useful for a better understanding of the interdependence of different traits at the QTL molecular level.

关 键 词：wheat （Triticum aestivum L.） gluten strength gluten index sedimentation volume unconditional QTL mapping conditional QTL mapping 

分 类 号：S512.1[农业科学—作物学] TS213.2[轻工技术与工程—粮食、油脂及植物蛋白工程]