淫羊藿苷联合胚胎干细胞对造影剂肾病大鼠模型肾上皮细胞凋亡的影响  

Effect of icariine combined with embryonic stem cells on apoptosis of kidney epithelial cells in contrast-induced nephropathy rat models

作　　者：赛依帕[1] 宋元春[1] 刘璟[1] 赵海燕 

机构地区：[1]甘肃省人民医院儿科,甘肃兰州730000

出　　处：《吉林大学学报（医学版）》2017年第5期953-957,共5页Journal of Jilin University：Medicine Edition

基　　金：甘肃省中医药管理局科研项目资助课题(GZK-2014-59)

摘　　要：目的:探讨淫羊藿苷联合胚胎干细胞对造影剂肾病(CIN)大鼠模型肾上皮细胞的作用及其可能的机制,为临床上淫羊藿苷联合应用提供实验依据。方法:选取SD大鼠50只,随机分为对照组、模型组、淫羊藿苷组、干细胞组和联合给药组,每组10只,除对照组外其他各组大鼠尾静脉注射吲哚美辛(INDO)、N-硝基-LO-精氨酸甲酯(L-NAME)和泛影葡胺建立CIN大鼠模型。检测造模前后血肌酐(Scr)值;采用半定量RT-PCR和Western blotting法检测细胞凋亡相关因子p38MAPK和caspase 3mRNA和蛋白的表达,流式细胞术定量检测肾上皮细胞凋亡率。结果:Scr测定,注射造影剂48h后,大鼠Scr值明显升高,提示造模成功。与模型组比较,联合给药组大鼠Scr值明显下降(P<0.05)。与模型组比较,淫羊藿苷组、干细胞组和联合给药组大鼠p38MAPK和caspase-3mRNA和蛋白表达水平降低(P<0.05);联合给药组大鼠肾上皮细胞凋亡率明显低于淫羊藿苷组和干细胞组(P<0.05)。结论:联合给药对CIN模型大鼠的肾上皮细胞有保护作用,其作用机制可能与抑制细胞凋亡通路中相关因子有关。Objective：To investigate the effect of icariin combined with embryonic stem cells on the renal epithelial cells in the contrast-induced nephropathy（CIN）rats and its possible mechanism,and to provide experimental evidence for clinical application of icariin.Methods：Fifty SD rats were randomly divided into,control group,model group,icariin group,stem cell group and combination group（n=10）.The rat models were established by tail vein injection of INDO,L-NAME and diatrizoate.The serum creatinine（Scr）levels of the rats were detected before and after modeling.The expressions of p38 MAPK and caspase-3 mRNA and protein were detected by semi-quantitative RT-PCR and Western blotting method.The apoptotic rate of renal epithelial cells was detected by flow cytometry.Results：The levels of Scr of rats were significantly increased 48 hafter combination contrast agent injection,suggesting that the CIN models were successfully set up.Compared with model group,the Scr level of the rats in group was significantly decreased（P〈0.05）.compared with model group the expression levels of p38 MAPK and caspase-3 mRNA and protein in icariin group and stem cell group and in combination group were decreased（P〈0.05）.The apoptotic rate of renal epithelial cells in combination group was significantly lower than those in icariine and stem cell groups（P〈0.05）.Conclusion：Icariine combined with embryonic stem cells has aprotectice effect on the renal epithelial cells in the CIN rats,and its mechanism may be related to the inhibition of the related factors in the apoptosis pathways.

关 键 词：淫羊藿苷 胚胎干细胞 造影剂肾病 肾上皮细胞 细胞凋亡 

分 类 号：R692[医药卫生—泌尿科学]