LH/EGF诱导ICR小鼠窦状卵泡卵母细胞体外成熟与排卵的研究  

LH/EGF induction of oocyte maturation and ovulation in cultured antral follicles of ICR mice

作　　者：尹耀学 苏友强[1] 

机构地区：[1]南京医科大学生殖医学国家重点实验室,江苏南京211166

出　　处：《南京医科大学学报（自然科学版）》2017年第9期1163-1167,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家重点基础研究发展计划(973计划)

摘　　要：目的 :利用医学研究常用ICR品系小鼠,在建立窦状卵泡体外培养体系的基础上,研究黄体生成素(luteinizing hormone,LH)与表皮生长因子(epidermal growth factor,EGF)诱导卵母细胞体外成熟及排卵的作用。方法 :体外剥离得到大的窦状卵泡用于培养并建立适宜于研究诱导卵母细胞成熟及排卵的窦状卵泡体外培养模型,通过向培养液中添加LH和EGF诱导卵母细胞体外成熟与排卵。结果:成功构建了ICR小鼠窦状卵泡体外培养模型,发现LH或EGF单独处理均可诱导卵泡卵母细胞成熟和卵丘扩展,而两者共同作用才能够诱导卵泡体外排卵。结论:LH/EGF促进ICR小鼠窦状卵泡卵母细胞体外成熟与排卵。Objective： To establish an antral follicle culture system suitable for investigating oocyte maturation and ovulation in ICR mice,a strain of mice that are frequently used in biomedical research in China,and to study the effect of luteinizing hormone （LH） and epidermal growth factor （EGF） on induction of oocyte maturation and ovulation in this follicle culture system.Methods： Large antral follicles were dissected and cultured in vitro,oocyte maturation and ovulation in these cultured follicles were then induced by supplementation of LH and EGF to the culture medium.Results： The antral follicle culture system suitable for investigating oocyte maturation and ovulation in ICR mice was successfully established.In this culture system,treatment with LH or EGF alone was able to induce oocyte maturation and cumulus expansion in follicle-enclosed cumulus-oocyte complexes,whereas supplementation with both LH and EGF was necessary for induction of ovulation.Conclusion： LH/EGF induces oocyte maturation and ovulation in cultured large antral follicles of ICR mice.

关 键 词：窦状卵泡 卵母细胞体外成熟 排卵 ICR小鼠 女性生殖 

分 类 号：R329.1[医药卫生—人体解剖和组织胚胎学]