高效液相色谱法测定天麻中4种化合物的含量  

HPLC Determination of 4 Compounds in Gastrodiaelata

作　　者：周美[1] 柳小兰[1] 张明[1] 曹佩雪[1] 潘卫东[1] 王道平[1] 曹永直 邵先强 

机构地区：[1]贵州省中国科学院天然产物化学重点实验室,贵阳550002 [2]德江县科技中心,德江565220 [3]德江县中药材产业办公室,德江565220

出　　处：《理化检验（化学分册）》2017年第10期1146-1150,共5页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：贵州省中药材现代农业产业技术体系建设项目(GZCYTX2015-0202);基于贵州特色药用资源的创新药物研发团队及平台建设(黔科合体Z字[2014]4007号);贵州省科技厅2015年度科研机构服务企业行动计划项目(黔科合服企[2015]4008号)

摘　　要：采用高效液相色谱法同时测定天麻中天麻素、对羟基苯甲醇、对羟基苯甲醛和巴利森苷的含量。样品(1.000 0 g)经60%(体积分数)乙醇40 mL于90℃回流提取1.0 h后进行色谱分离,以Syncronis C18色谱柱为固定相,以不同体积比的甲醇和0.05%(体积分数)磷酸溶液的混合液为流动相进行梯度洗脱。4种化合物的进样质量在一定范围内与其峰面积呈线性关系,检出限(3S/N)在0.003~0.012μg之间。按标准加入法进行回收试验,回收率在96.4%~98.4%之间,测定值的相对标准偏差(n=6)均小于1.5%。HPLC was applied to the determination of gastrodin, 4-hydroxybenzyl alcohol, 4- hydroxybenzaldehyde and parishin in Gastrodiaelata. The sample （1. 000 0 g） was extracted by reflux at 90 ℃ for 1.0 h with 40 mL of 60% （φ） ethanol, and then the solution was separated by HPLC using Syncronis C18 column as stationary phase and a mixture of methanol and 0. 05% （φ） phosphoric acid solution in various ratios as mobile phase in gradient elution. Linear relationships between values of peak area and injection mass of 4 compounds were found in definite ranges, with detection limits （3S/N） in the range of 0. 008-0. 012 μg. Test for recovery was made by standard addition method, giving results in the range of 96.4%-98. 4%, with RSDs （n=6） all less than 1.5%.

关 键 词：高效液相色谱法 天麻 天麻素 对羟基苯甲醇 对羟基苯甲醛 巴利森苷 

分 类 号：O652.63[理学—分析化学]