极谱法测定环境水中四环素的含量  

Polargraphy Determination of Tetracycline in Environmental Water

作　　者：孙雪梅 高娅玲[1,2] 陈文 刘志[1] 

机构地区：[1]成都理工大学材料与化学化工学院,成都610059 [2]建水实验中学,建水654300

出　　处：《理化检验（化学分册）》2017年第10期1156-1160,共5页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：以十六烷基三甲基溴化铵(CTMAB)对四环素(TC)极谱峰的增敏作用为基础,提出了测定TC含量的极谱法。优化的试验条件如下:CTMAB-Na2HPO4-Na H2PO4缓冲溶液(p H 7.7)为底液,扫描速率0.7 V·s-1,起始电位-0.85 V,静止时间6 s,温度25~30℃。在上述试验条件下,TC的线性范围为0.02~1.0 mg·L^(-1),1.0~80mg·L^(-1),检出限(3s/k)为0.001 5 mg·L^(-1)。测定值的相对标准偏差(n=6)均小于4.0%。在3个浓度水平上进行加标回收试验,测得回收率在92.0%~120%之间。A method for determination of tetracycline （TC） by polargraphy was proposed, which was based on the sensitization effect of cetyl trimethyl ammonium bromide （CTMAB） on the polarographic peak of TC. The optimized experimental conditions were as follows： ① a mixture of CTMAB and Na2HPO4-NaH2PO4 buffer solution （pH 7. 7） was used as the base solution; ② the scanning rate was 0. 7 V ·s^-1 ;③the starting potential was -0. 85 V;④ the stationary time was 6 s;⑤ the temperature was 25- 30 ℃. In above experimental conditions, linearity ranges for mass concentration of TC found were 0. 02-1.0 mg ·L^-1 and 1.0-80 mg·L^-1. Values of detection limit （3s/k） found was 0. 001 5 mg ·L^-1 , and values of RSDs （n=6） found were both less than 4. 0%. Test for recovery was made by standard addition method at 3 concentration levels, giving results in the range of 92.0%-120%.

关 键 词：极谱法 四环素 环境水 十六烷基三甲基溴化铵 缓冲溶液 

分 类 号：O657.14[理学—分析化学]