DNA免疫技术在高质量单克隆抗体诱导开发中的应用进展  

Progress in Application of DNA Immunization Technique in R&D of High-quality Monoclonal Antibodies

作　　者：刘淑颖 陈雨欣 王世霞[1] 卢山[1] 

机构地区：[1]美国马萨诸塞大学医学院内科系,马萨诸塞州伍斯特01605 [2]SYL咨询公司,加利福尼亚州干橡91320 [3]南京大学医学院附属鼓楼医院检验科,江苏南京210008

出　　处：《药学进展》2017年第9期662-669,共8页Progress in Pharmaceutical Sciences

摘　　要：近年来研究发现,DNA疫苗能有效诱导机体产生高质量的抗体反应。深入探讨了DNA免疫技术诱导宿主免疫反应尤其是抗原特异性抗体反应的分子机制,并介绍如何将这一技术应用到单克隆抗体的诱导开发,包括DNA疫苗构建、递送方式、分子佐剂和DNA初免-蛋白质加强免疫等有关具体技术要点。Recent research advances have suggested that DNA vaccines are effective in inducing and generating of high-quality antibody responses. In this review, we provided in-depth update on the molecular mechanisms involved in the induction of immune responses especially antigen specific antibody responses by DNA immunization technique, and introduced how to apply this technique to the research and development of monoclonal antibodies, including key technical steps related to DNA vaccine design, delivery method, molecular adjuvants, and DNA prime-protein boost immunization strategy.

关 键 词：DNA免疫 单克隆抗体 DNA疫苗 

分 类 号：R392[医药卫生—免疫学]