去甲斑蝥素温敏型原位凝胶的制备及其对肝癌的抑制作用研究  

Study on Preparation and Anti-hepatoma of Norcantharidin Thermosensitive In-situ Gel

作　　者：谢明华[1] 蔡鑫君[2] 彭佳蓓[1] 蒋晓蕊 葛敏[1] 

机构地区：[1]杭州市余杭区第一人民医院药剂科,杭州311100 [2]浙江省中西医结合医院药剂科,杭州310003

出　　处：《中国现代应用药学》2017年第9期1262-1265,共4页Chinese Journal of Modern Applied Pharmacy

基　　金：杭州市科技计划项目(20140633B57);杭州市医药卫生科技计划(2015B32201552416);杭州市余杭区科技计划项目(2014003)

摘　　要：目的制备去甲斑蝥素温敏型原位凝胶并探讨其对肝癌的抑制作用。方法以泊洛沙姆407及泊洛沙姆188为载体材料制备去甲斑蝥素温敏型原位凝胶,采用小鼠H22肝癌细胞实体瘤模型考察去甲斑蝥素温敏型原位凝胶对荷瘤小鼠的肿瘤生长抑制作用。结果制备所得原位凝胶在室温下外观为白色透明液体态,胶凝温度下发生相转变形成凝胶时外观为白色透明凝胶态,凝胶温度为34℃,具有显著的缓释特性;体内抗肿瘤活性实验研究表明,对小鼠荷肝癌实体瘤具有明显抑制作用。结论去甲斑蝥素温敏型原位凝胶性质稳定,并具有良好的抗肿瘤活性。OBJECTIVE To prepare thermosensitive in-situ gel of norcantharidin（NCTD） and to explore its inhibitory effects on hepatoma. METHODS NCTD thermosensitive in-situ gel was prepared using poloxamer 407 and poloxamer 188. Tumor growth inhibition caused by NCTD thermosensitive in-situ gel was examined on tumor-bearing mice using mouse H22 hepatoma cells to build a solid tumor model. RESULTS The prepared in-situ gel displayed a white transparent liquid state at room temperature but a white transparent gel state at gelling point, which was 34 ℃. Significant slow release characteristic was presented at gel state; anti-tumor activity experiment in vivo suggested significant inhibition effects on tumor growth in solid hepatoma bearing mice. CONCLUSION NCTD thermosensitive in-situ gel owns stable properties and good antitumor activities.

关 键 词：去甲斑蝥素 温敏型原位凝胶 肿瘤抑制 

分 类 号：R944.1[医药卫生—药剂学]