Box-Behnken Design-响应面法优化绿原酸转化为新绿原酸的工艺研究  

Optimization on Transformation from Chlorogenic Acid to Neochlorogenic Acid by Box-Behnken Design-response Surface Methodology

作　　者：温建辉[1] 倪付勇[1] 王雪晶[1] 李明[1] 宋亚玲[1] 王振中[1] 萧伟[1] 

机构地区：[1]江苏康缘药业股份有限公司,连云港222000

出　　处：《世界科学技术-中医药现代化》2017年第7期1239-1244,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：科学技术部国家"重大新药创制"科技重大专项(2013ZX09402203):现代中药创新集群与数字制药技术平台;负责人:王振中

摘　　要：利用Box-Behnken-响应面法优化绿原酸转化为新绿原酸的工艺条件。在单因素实验基础上,采用Box-Behnken Design实验设计,考察反应温度、反应时间及p H值对新绿原酸产率的影响,优化绿原酸转化为新绿原酸的工艺参数,得到模型预测公式。优化条件为:反应温度107℃,反应时间60 min,p H值为4.72时新绿原酸产率为64.20%,与模型预测值接近,方程拟合良好。采用优选工艺制备新绿原酸并精制纯化,分别运用HPLC、1H-NMR、13C-NMR、ESI-MS等方法对其进行含量测定和结构表征,结果表明纯化后纯度达98.78%,收率为87.37%。Box-Behnken design-response surface methodology was used to optimize the transformation from chlorogenicacid to neochlorogenic acid. Based on single factor experiments, the experiment design were developed by box-benhnhencentral composite design with causal factors of the reaction temperature, time and PH to neochlorogenic acid. Theoptimum transformation conditions were as follow： reaction temperature at 107°C, reaction time of 60 min, the PH of4.72. Under the optimum extraction technology conditions, the productivity of neochlorogenic acid was 64.20%.Neochlorogenic acid was isolated and purified. The determination and characterization of neochlorogenic acid wasdetected by HPLC, 1H-NMR, 13C-NMR and ESI-MS. The results showed that the content of neochlorogenic acidreached to 98.78% and the yield of 87.37%.

关 键 词：绿原酸 新绿原酸 Box-Behnken Design响应面法 结构鉴定 

分 类 号：R284.1[医药卫生—中药学]