桑叶浸出液抑制MDA-MB-231细胞增殖和抑制Survivin蛋白表达  

Inhibitory Effects of Mulberry Leaf Leachates on Proliferation of MDA-MB-231 Cells and Their Survivin Protein Expression

作　　者：缪伟伟 王志雄[1,3] 石仝雨 大野優子[2] 

机构地区：[1]上海健康医学院药学院,上海200237 [2]日本国立大阪大学医学系研究科 [3]上海理工大学医疗器械与食品学院,上海200093

出　　处：《现代食品科技》2017年第9期13-19,共7页Modern Food Science and Technology

基　　金：国家自然科学基金资助项目(81402030)

摘　　要：为了研究桑叶浸出液对乳腺癌MDA-MB-231细胞增殖和对凋亡抑制蛋白Survivin的作用。本实验采用体外培养MDA-MB-231细胞,倒置显微镜观察桑叶浸出液作用后细胞形态变化;CCK-8法检测桑叶浸出液对MDA-MB-231细胞增殖的影响;流式细胞仪分析桑叶浸出液对MDA-MB-231细胞凋亡及周期的影响;Western Blot印迹法检测桑叶浸出液对凋亡抑制蛋白Survivin表达的影响。结果表明桑叶浸出液会引起MDA-MB-231生长形态的变化,抑制细胞增殖,增加细胞凋亡数量,且在桑叶浸出液作用后MDA-MB-231细胞的G0/G1期细胞明显高于正常组(p<0.05),同时桑叶浸出液降低凋亡抑制蛋白Survivin的表达,且表达量随着桑叶浸出液浓度的升高而降低。研究表明,桑叶浸出液能够抑制MDA-MB-231细胞的增殖,促进细胞凋亡及导致G0/G1期阻滞,同时能够降低凋亡抑制蛋白Survivin的表达。The aim of this paper was to study the effects of mulberry leaf leachates on the proliferation of MDA-MB-231 breast cancer cells and antiapoptotic protein survivin. MDA-MB-231 cells were cultured in vitro, and the morphological changes were examined under an inverted microscope. The effect of the mulberry leaf leachates on the cell proliferation was detected by the cell counting kit-8 （CCK8） method. The influence of the mulberry leaf leachate on apoptosis and changes in the cell cycle of MDA-MB-231 cells were analyzed by flow cytometry. Finally, the effect of the mulberry leaf leachates on the expression of the survivin protein was detected by western blotting. The results showed that the leachate of mulberry leaves significantly changed the growth and morphology of MDA-MB-231 cells： cell proliferation was inhibited, and the number of apoptotic cells increased significantly. After thetreatment with the mulberry leaf leachate, the number of MDA-MB-231 cells at the G0/G1 transition was significantly higher than that of the normal group （p 〈 0.05）. At the same time, the protein expression of survivin in the mulberry leaf leachate group decreased with the increasing concentration of the mulberry leaf leachate as compared with that of the control group. The results showed that the mulberry leaf leachate may inhibit proliferation of MDA-MB-231 cells, promote apoptosis, lead to G0--G1 arrest, and downregulate the survivin gene.

关 键 词：桑叶浸出液 细胞增殖 凋亡抑制蛋白SURVIVIN 

分 类 号：R285[医药卫生—中药学]