水黄皮HPLC指纹图谱的优化及建立  

Optimization of conditions and establishment of HPLC fingerprint of Pongamia pinnata

作　　者：陈露[1] 张晓萌[1] 陈国彪[1] 林秋婕 张海防[1] 陈赞民 

机构地区：[1]海南省药品检验所,海南海口570216

出　　处：《中国热带医学》2017年第10期966-969,974,共5页China Tropical Medicine

基　　金：海南省自然科学基金(No.311047)

摘　　要：目的通过筛选试样处理方法和优化水黄皮高效液相色谱分析方法,建立水黄皮的指纹图谱。方法实验比较了三种不同的提取方法和提取时间,考察了不同型号色谱柱的分离效果和多种流动相系统的梯度洗脱能力,优化了检测波长、柱温和流速等分析条件。色谱柱为Agilent ZORBAX SB C18(250 mm×4.6 mm,3.5μm)柱,流动相为乙腈(A)-0.5%H3PO4(B)溶液,梯度洗脱;检测波长为303 nm;柱温为35℃;流速为1.0 m L/min。采用中药色谱指纹图谱相似度评价系统软件(2004 A版)进行指纹图谱分析。结果通过优化检测波长、流动相、洗脱程序等建立了水黄皮HPLC指纹图谱及共有模式,标定9个共有峰。9批样品的相似度为0.828~0.983。不同来源的水黄皮指纹图谱基本一致,所含化学成分的种类和含量较稳定。结论该方法相对于单一成分的质量控制模式,能够直观、全面地从整体上反映水黄皮药材的质量,准确可靠、重复性好,为水黄皮的鉴定和质量控制提供了参考依据。Objective To establish the fingerprint of Pongamia pinnata by screening the samples and optimizing thehigh performance liquid chromatography（HPLC） analysis method. Methods Three different extraction methods andextraction time were compared. The separation effect of different types of columns and the gradient elution ability of variousmobile phase systems were investigated, and the analysis conditions such as detection wavelength, column temperature andflow rate were optimized. The HPLC method was performed with Agilent ZORBAX SB C18 column（250 mm×4.6 mm, 3.5 μm）,and the mobile phase was acetonitrile（A）-0.5% H3PO4（B）（gradient elution） with a flow rate of 1.0 m L/min. The detectionwavelength was 303 nm, and the column temperature was 35 ℃. The Similarity Evaluation System for ChromatographicFingerprint of Traditional Chinese Medicine（2004 A edition） was used to analyze the results. Results The HPLC fingerprintof P. pinnata and its common mode were established with the optimization of the detection wavelengths, mobile phase andelution conditions. Nine common peaks were characterized. The similarities of nine batches were 0.828-0.983. The fingerprintprofiles of various samples from different regions were basically identical, with the relatively stable type and content of thechemical compositions. Conclusion Compared with the single component quality control mode, this method can reflect thequality of P. pinnata in an intuitive and comprehensive way, and it is accurate, reliable and reproducible, which provides thereference for the identification and quality control of P. pinnata.

关 键 词：水黄皮 指纹图谱 高效液相色谱法 质量控制 

分 类 号：R284.1[医药卫生—中药学]