电针联合跑台训练对大鼠骨骼肌钝挫伤的修复作用  

The Effect of Electroacupuncture Combined with Treadmill Training on Skeletal Muscle Contusion in Rats

作　　者：张安宁[1] 黄思琴[1] 唐成林[1] 罗翱[1] 赵丹丹[1] 郭全虎[1] 高睿琦 曹净[1] 

机构地区：[1]重庆医科大学中医药学院,重庆400016

出　　处：《针刺研究》2017年第5期391-396,共6页Acupuncture Research

基　　金：国家自然科学基金项目(No.81403466;81273870);重庆市渝中区项目(No.20140117);重庆市卫计委项目(No.ZY 20150250);重庆市教委项目(No.KJ 1702033);重庆市体育局项目(No.B 201408)

摘　　要：目的:观察电针联合跑台训练对大鼠骨骼肌钝挫伤的修复作用,探讨电针联合跑台训练改善骨骼肌损伤的可能机制。方法:SD大鼠随机分为正常组、模型组、电针组、跑台组和联合组,每组12只。采用自制打击装置建立骨骼肌钝挫伤模型。电针组电针"足三里"、阿是穴,跑台组进行跑台训练,联合组同时进行电针和跑台训练,1次/d,连续至损伤后3、14d。HE染色观察损伤后14d腓肠肌新生肌细胞横截面积和直径,Western blot法检测损伤后3d腓肠肌哺乳动物雷帕霉素靶蛋白(mTOR)、肌细胞生成素(myoG)和14d腓肠肌mTOR、快肌型骨骼肌肌球蛋白重链(Fast MyHC)的表达量。结果:模型组新生肌细胞横截面积和直径明显小于正常组(P<0.01),各治疗组均显著大于模型组(P<0.05),联合组显著大于电针组和跑台组(P<0.05)。与正常组比较,损伤后3d模型组腓肠肌mTOR和myoG蛋白表达均显著升高(P<0.01);与模型组比较,各治疗组腓肠肌mTOR和myoG蛋白表达均显著升高(P<0.01);与电针组比较,跑台组腓肠肌mTOR和myoG蛋白表达均显著降低(P<0.01);与电针组和跑台组比较,联合组腓肠肌mTOR和myoG蛋白表达显著升高(P<0.05,P<0.01)。与正常组比较,损伤后14d模型组腓肠肌mTOR蛋白表达升高,但差异无统计学意义(P>0.05),Fast MyHC蛋白表达显著降低(P<0.01);与模型组比较,各治疗组腓肠肌mTOR及Fast MyHC蛋白表达均显著升高(P<0.05,P<0.01);与电针组比较,跑台组腓肠肌mTOR及Fast MyHC蛋白表达均显著降低(P<0.05,P<0.01);与电针组和跑台组比较,联合组腓肠肌mTOR及Fast MyHC蛋白表达均显著升高(P<0.01)。结论:电针联合跑台训练能促进成肌细胞分化成熟,减轻骨骼肌损伤,该作用与增加mTOR表达量,正向调控myoG、Fast MyHC的表达有关。Objective To study the effect and mechanism of electroacupuncture（EA）combined with treadmill training in rats with skeletal muscle contusion. Methods A total of sixty Sprague Dawley rats were randomly divided into normal,model, EA, treadmill, and combination groups （n = 12/group）. The self-made striking device was used to establish skeletal muscle contusion model. EA was applied at ＂Zusanli＂ （ST 36） and Ashi point in the EA group. Treadmill was applied to train rats in the treadmill group. Rats in the combination group were received the above two methods. All the treatment was given once a day until the 3^rd and 14^th days after injury. The cross-sectional area and diameter of neonatal gastrocnemius muscle cells 14 days after injury were observed by HE staining. The expression levels of mammalian target of rapamycil （mTOR）, myogenin （myoG） in gastrocne- mius 3 days after injury and gastrocnemius mTOR, Fast myosin skeletal heavy chain （Fast MyHC） 14 days after injury were observed by Western blot. Results The cross-sectional area and diameter of neonatal muscle gastrocnemius cells in the model group were significantly lower than those in the normal group （P〈0.01）, and those in all the intervention groups were significantly higher than those of the model group （ P〈0.05）, with better results in the combination group compared with those in the EA and treadmill groups （ P〈0.05）. On the 3^rd day after injury, the expressions of mTOR and myoG proteins in the model group were sig- nificantly higher compared with those in the normal group （P〈0.01）, and those in all the intervention groups were up-regulated in comparison with the model group （all P〈0.01 ）. The mTOR and myoG proteins in the treadmill group were lower than those in the EA group （P〈0.01）. The above two indexes in the combination group were higher than those in the EA and treadmill groups （P〈0.05, P〈0.01）. On the 14^th day after injury, the expression of mTOR was up-regulated in the model

关 键 词：骨骼肌钝挫伤 电针联合跑台 哺乳动物雷帕霉素靶蛋白 肌细胞生成素 快肌型骨骼肌肌球蛋白重链 

分 类 号：R245.97[医药卫生—针灸推拿学]