电针“足三里”穴对腹泻型肠易激综合征大鼠平滑肌收缩骨架蛋白——波形蛋白的影响  

Effect of Electroacupuncture at“Zusanli”(ST 36)on Vimentin (A Kind of Cytoskeleton Protein Related to Smooth Muscle Contraction) in Rats with Diarrhea-predominant Irritable Bowel Syndrome

作　　者：杨大业 王华 李佳 吴松 刘建民 王丹 王丽华 

机构地区：[1]湖北中医药大学针灸骨伤学院针灸治未病湖北省协同创新中心,武汉430061

出　　处：《针刺研究》2017年第5期402-406,共5页Acupuncture Research

基　　金：湖北省自然科学基金课题(No.2016CFB 221);湖北中医药大学校级课题(No.XJ 2014KJ 003)

摘　　要：目的:通过电针"足三里"穴干预腹泻型肠易激综合征(IBS-D)大鼠,探讨其对平滑肌细胞骨架蛋白——波形蛋白(Vimentin)的影响。方法:SD大鼠随机分为空白组、模型组、电针组、匹维溴铵组,每组15只。采用高乳糖饲料喂养,结合慢性不可预知温和刺激法制备IBS-D大鼠模型。观察各组大鼠体质量变化、胃排空及小肠推进率;HE染色观察结肠组织形态学改变;免疫组化法检测结肠组织中Vimentin蛋白的表达。结果:与空白组相比,模型组大鼠体质量、小肠推进率、Vimentin蛋白表达水平显著升高(P<0.05),胃排空率显著降低(P<0.05)。经治疗后,电针组和匹维溴铵组大鼠体质量及小肠推进率较模型组显著下降(P<0.05),电针组胃排空率显著升高、Vimentin蛋白表达水平显著降低(P<0.05)。结论:电针"足三里"穴可以下调结肠Vimentin蛋白的表达,从而改善IBS-D大鼠胃肠动力,对平滑肌细胞收缩具有良好的调节作用。Objective To investigate the effect of electroacupuncture （EA） at ＂Zusanli＂ （ST 36） on cytoskeleton protein-Vimentin （related to smooth muscle contraction） in rats with diarrhea-predominant irritable bowel syndrome （IBS-D）. Methods Sixty female Sprague-Dawley （SD） rats were randomly divided into normal, model, EA and pinaverium bromide （PB） groups （n = 15/group）. The IBS-D model was established by the method of high lactose feeding and chronic unpredictable mild stress. The changes of body weight, gastric emptying and small intestine propulsion rates were observed. The morphological changes of colon were observed by HE staining. And Immunohistochemical method was used to detect the expression of Vimentin pro- tein in colon. Results The body weight, small intestine propulsion rate and expression of Vimentin protein of the model group were significantly higher than those of the normal group （all P〈0.05）, while the gastric emptying rate decreased （ P〈0.05）. After treatment, the body weight and small intestine propulsion rate of the EA group and PB group decreased compared with those of the model group （all P〈0.05）. The gastric emptying rate was higher and the expression of Vimentin protein was lower in the EA group than those in the model group （both P〈0.05）. Conclusion EA at ST 36 can down-regulate the expression of Vimentin protein in colon, thus improving the gastrointestinal motility and regulating the contraction of smooth muscle cells of IBS rats.

关 键 词：肠易激综合征 电针 足三里穴 平滑肌收缩 

分 类 号：R245.97[医药卫生—针灸推拿学]