宜都周边养殖水体及鱼肉中阿维菌素含量测定  

Determination of Avermectin in the Pond Water and Fish from the Aquaculture Areas in Yidu

作　　者：胡飞飞 陈良立 刘珊 黄悦 余胜男 陈龙 乔曦 邓张双 杜维力[2] 付彩霞 Hu Feifei Chen Liangli Liu Shan Huang Yue Yu Shengnan Chen Long Qiao Xi Deng Zhangshuang Du Weili Fu Caixia(Hubei Key Laboratory of Natural Products Research ＆ Development, College of Biological ＆ Pharmaceutical Sciences, China Three Gorges Univ. , Yichang 443002, China Research ＆ Development Center, Angel Yeast Co. , Ltd. , Yichang 443003, China Hubei Old Silly Soil Ecological Agriculture Science Technology Co. , Ltd. , Yidu 443302, China)

机构地区：[1]三峡大学生物与制药学院天然产物研究与利用湖北省重点实验室,湖北宜昌443002 [2]安琪酵母股份有限公司研发中心,湖北宜昌443003 [3]湖北土老憨生态农业科技股份有限公司,湖北宜都443302

出　　处：《三峡大学学报（自然科学版）》2017年第5期109-112,共4页Journal of China Three Gorges University：Natural Sciences

基　　金：国家自然科学基金项目(311000801);三峡大学教学研究重点项目(No.J2015037);生物与制药学院大学生科技创新课题

摘　　要：建立固相萃取-高效液相色谱测定养殖水体及鱼类中阿维菌素(Avermectins)残留量的方法,了解AVMs在宜都水产养殖中的使用情况.采用固相萃取法(Solid Phase Extraction,SPE)对水体和鱼类中AVMs进行富集,高效液相色谱法(High Performance Liquid Chromatography,HPLC)进行含量检测,外标法定量.结果发现,AVMs在2.0~128.0μg/mL质量浓度范围内呈线性关系,相关系数为0.999 9.测定结果显示:在宜都周边养殖区所采集的6份水样和7份鱼样中均检测到AVMs的残留,残留量分别为0.004 4~0.127 7mg/kg和0.031 7~1.069 9mg/kg,高于日本《食品中残留农业化学品肯定列表制度》中规定鱼肉中最大残留限量0.005mg/kg.固相萃取与HPLC法联合运用能精确测定水体及鱼类中AVMs的含量,样品处理方法简单、快速,实用性强.To investigate the usage of avermectin in aquaculture areas in Yidu, a method for the determination of avermectin residues in aquaculture water and fish by solid phase extraction and high performance liquid chromatography （HPLC） was established. The avermectin was enriched in aquaculture water and fish by solid phase extraction （SPE） and analyzed by high performance liquid chromatography （HPLC） with external standard method. The avermectin concentration and peak area showed a good linear relationship in the range of 2--128 ~g/mL and the correlation coefficient was 0. 999 9. The analytical results showed that the residues of avermectin in the water and fish samples were 0.004 4--0. 127 7 mg/kg and 0. 031 7--1. 069 9 mg/kg, re- spectively, which were much higher than the maximum residue limit in the Japanese Positive List System of 0. 005 mg/kg. The combination of solid phase extraetion and HPLC could be used for accurate determination of avermectin residues in water and fish. The method of sample treatment is simple, rapid and practical.

关 键 词：阿维菌素 抗生素 固相萃取 高效液相色谱法 水产养殖 

分 类 号：S948[农业科学—水产养殖]