酶联免疫法检测小儿乙肝PreS1抗原及抗体在乙肝诊断中的应用价值  

Clinical value of enzyme-linked immunosorbent assay in detecting hepatitis B PreS1 antigen and antibody in children

作　　者：唐莲[1] 朱咏贵[1] 张慧[1] 唐艳 

机构地区：[1]湖南省儿童医院肝病中心,湖南长沙410007 [2]湖南省邵阳市中心医院检验科,湖南邵阳422000

出　　处：《实用检验医师杂志》2017年第3期146-149,共4页Chinese Journal of Clinical Pathologist

摘　　要：目的探讨酶联免疫法检测小儿乙肝病毒前S1蛋白(PreS1)抗原及抗体在小儿乙肝诊断中的应用价值。方法在2014年至2016年在本院接受诊断和治疗的乙肝患儿中,选择200例资料完整且接受两对半检查和酶联免疫法检测血清PreS1抗原及抗体的患儿作为研究对象,计算不同两对半检测结果患儿PreS1抗原及抗体阳性率,分析PreS1抗原阳性与HBV-M的Spearman相关性。结果 200例乙肝患者入选。18例乙肝大三阳患儿的PreS1抗原阳性及抗体阳性的检出率较高,均为83.33%。25例PreS1抗原阳性组患儿与175例抗原阴性组患儿HBsAg、HBeAg和HBcAb数据对比差异具有统计学意义,说明PreS1抗原阳性与HBsAg、HBeAg和HBcAb具有正相关关系,相关数值分别为0.620、0.550、0.720,P值分别为0.021、0.009、0.020。结论乙肝PreS1抗原及抗体阳性检测准确率相对较高,可以作为乙肝诊断的补充检验,为患儿的临床诊断和治疗提供依据。Objective To investigate the value of enzyme linked immunosorbent assay （ELISA） in detecting hepatitis B PreS1 antigen and antibody in children. Methods From 2014 to 2016 in our hospital 200 cases of children with incomplete information and accept the two semi inspection and enzyme-linked immunosorbent assay of serum examination for diagnosis and treatment of hepatitis B were selected as the research object. Positive rates of PreS1 antigen and antibody, and Spearman correlation between PreS1 antigen positive and HBV-M were calculated. Results Two hundred cases of hepatitis B patients were enrolled. Eighteen patients with HBeAg positive PreS1 antigen and antibody positive detection rate was higher, more than 83.33% cases. Twenty-five PreS1 antigen positive group and 175 cases of children with HBsAg antigen were negative group, the difference was statistically significant in HBeAg and HBcAb data contrast, the PreS1 antigen positive and HBsAg, HBeAg and HBcAb had positive correlation, with correlation values were 0.620, 0.550, 0.720 and P = 0.021, 0.009, 0.020. Conclusion The detection accuracy of hepatitis B PreS1 antigen and antibody is relatively high, which can be used as a supplementary test for hepatitis B diagnosis, and provide the basis for clinical diagnosis and treatment of children.

关 键 词：酶联免疫法 乙肝PreS1 抗原 抗体 诊断 

分 类 号：R446.6[医药卫生—诊断学] R725.1[医药卫生—临床医学]