妊娠期胆汁淤积症孕妇胎盘组织TP63的表达及其与血高胆酸的相关性  

Expression of placental tissue TP63 in pregnant women with ICP and its correlationship with excessive bile acid

作　　者：丁晨 吴维宾[1] 蔡眉[2] 张慧娟[1] 

机构地区：[1]上海交通大学医学院附属国际和平妇幼保健院病理科&生物样本库,上海200030 [2]上海交通大学医学院附属国际和平妇幼保健院检验科,上海200030

出　　处：《中国临床医学》2017年第4期560-564,共5页Chinese Journal of Clinical Medicine

基　　金：上海市卫生和计划生育委员会面上项目(201640033)~~

摘　　要：目的:检测妊娠期肝内胆汁淤积症(ICP)及正常妊娠孕妇胎盘组织TP63的表达,并探讨其与血胆酸水平的相关性。方法:选择2012年1月至2014年12月在上海交通大学医学院附属国际和平妇幼保健院分娩的ICP患者70例(ICP组)及正常妊娠孕妇44例(对照组)作为研究对象。采用免疫组化法评估两组孕妇胎盘组织TP63的表达水平。采用RT-qPCR检测不同浓度胆汁酸作用对滋养细胞株JAR细胞TP63mRNA表达水平的影响。结果:ICP组患者胎盘滋养细胞(绝大部分为细胞滋养细胞)中TP63表达低于对照组,差异有统计学意义(P<0.05)。胎盘组织中TP63的表达与孕妇血清胆酸水平负相关(r=-0.679,P<0.001)。高浓度胆汁酸可引起JAR细胞中TP63 mRNA的表达量下降(P<0.05)。结论:高胆酸引起的胎盘滋养细胞中TP63低表达可通过减弱细胞增殖能力参与ICP发生发展。Objective：To compare the expression of TP63 in placentas from intrahepatic cholestasis of pregnancy（ICP）patients and normal pregnant women,and to discuss the relationship between bile acid and TP63. Methods：Retrospective study was conducted on 70 placentas from ICP patients（ICP group）and 44 placentas from normal term pregnancy（control group）.The samples were collected from January 2012 to December 2014 in the International Peace Maternity Child Health Hospital Affiliated to Shanghai Jiao Tong University School of Medicine.Immunohistochemistry assay was applied to evaluate the protein expression of TP63 in placentas of the two groups.In addition,the mRNA level of TP63 was tested by RT-qPCR assay in trophoblastic cell line JAR after pretreating with different concentrations of bile acids.Results：TP63 was majorly localized in cytotrophoblasts.The expression level of TP63 in placentas from ICP group were significantly lower than that in the control group（P〈0.05）.Moreover,the expression of TP63 was negatively correlated with total bile acid level in maternal serum at delivery（r=-0.679,P〈0.001）.Treatment with high concentration of bile acids reduced the mRNA level of TP63 in JAR cells.Conclusions：Down regulation of TP63 by excessive bile acids in placental trophoblasts would contribute to the progression of ICP possibly by attenuating the proliferation of trophoblasts.

关 键 词：妊娠期肝内胆汁淤积症 胎盘 TP63 免疫组织化学 胆酸 

分 类 号：R714.255[医药卫生—妇产科学]