17β-雌二醇对大鼠脂肪源干细胞增殖及胶原蛋白合成影响研究  

Effect of 17β-estrogen on adipose-derived stem cells proliferation and collagen synthesis in rats

作　　者：王苏[1] 杜培[1] 陈礼全[1] 张晓薇[1] WANG Su DU Pei CHENG Li-quan ZHANG Xiao-wei.(The First Affiliated Hospital of Guangzhou Medical University, Guangzhou ,510120, China)

机构地区：[1]广州医科大学附属第一医院妇产科,广东广州510120

出　　处：《中国实用妇科与产科杂志》2017年第10期1069-1073,共5页Chinese Journal of Practical Gynecology and Obstetrics

基　　金：广东省产业技术研究与开发资金计划项目(2012B031800247)

摘　　要：目的探讨雌激素对大鼠脂肪源干细胞(adipose-derived stem cells,ADSCs)增殖及Ⅰ、Ⅲ型胶原蛋白表达的影响。方法 2016年12月至2017年5月以广州医科大学第一附属医院保存的大鼠ADSCs作为研究对象,体外培养过程中加入不同浓度17-β雌二醇,MTT检测增殖能力,荧光定量PCR及蛋白质印记技术(Western blot)检测Ⅰ、Ⅲ型胶原蛋白mRNA表达及蛋白的表达情况。结果 17-β雌二醇浓度为10-9mol/L时,大鼠ADSCs增殖指数及Ⅰ、Ⅲ型胶原蛋白的表达明显增加,差异有统计学意义(P<0.05)。结论 17-β雌二醇可以促进ADSCs胶原蛋白的表达,两者联合应用有望成为治疗绝经后盆腔器官脱垂的方法之一。Objective To study the effect of 17β-estrogen on cell proliferation and collagen protein expression in rat ADSCs. Methods Between Dec.2016 and May 2017,the rat ADSCs,which was preserved in the First Affiliated Hospi- tal of Guangzhou Medical University, were used as the research cells and treated by different final concentrations of 17β- estrogen.Then, proliferation of ADSCs was determined by MTr assay and the expressions of collagen type I , III gene mRNA and protein were detected by quantitative real-time PCR and Western blot.Results When the concentration of 1713-estrogen was 10-μmol/L, the proliferation of ADSCs, the expression of type I and III collagen were increased, and the difference was statistically significant（P〈0.05）.Conclusion 17β-estrogen can promote the expression of collagen in rat ADSCs, and the combination of estrogen and ADSCs is expected to be one of the ways to treat postmenopausal pa- tients with pelvic organ prolapse.

关 键 词：盆底功能障碍性疾病 大鼠脂肪源干细胞 17Β-雌二醇 Ⅰ型胶原蛋白 Ⅲ型胶原蛋白 

分 类 号：R711.2[医药卫生—妇产科学]