高丹草AFLP分子遗传连锁图谱的构建  

Construction of AFLP molecular genetic linkage map of Sorghum-Sudangrass

作　　者：石悦[1] 于肖夏[1] 于卓[1] 杨东升[1] 姜超[1] 张明飞[1] SHI Yue YU Xiaoxia YU Zhuo YANG Dongsheng JIANG Chao ZHANG Mingfei(Agronomy College, Inner Mongolia Agriculture University, Huhhot, Inner Mongolia 010019, China)

机构地区：[1]内蒙古农业大学农学院,内蒙古呼和浩特010019

出　　处：《西北农林科技大学学报（自然科学版）》2017年第10期7-14,共8页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家"973"计划项目(2014CB138703);国家自然科学基金面上项目(31472141)

摘　　要：【目的】对高丹草低氢氰酸含量及产量等重要性状进行QTL定位,构建其AFLP分子遗传连锁图谱,为分子标记辅助育种等研究奠定基础。【方法】以散穗高粱×红壳苏丹草杂种F2代305个分离单株为作图群体,利用AFLP分子标记技术先从供试AFLP引物中筛选适宜引物对,再用这些引物对对其供测群体进行PCR扩增;根据扩增原始数据,利用Join Map 3.0作图软件进行高丹草分子遗传连锁图谱构建。【结果】从113对AFLP引物组合中筛选出了25对多态性丰富、重复性好、条带清晰的适宜引物,对高丹草F2分离群体305个单株的基因组DNA扩增,得到444个AFLP分子标记位点,据此构建的高丹草遗传图谱包含10个连锁群,覆盖的基因组总长度为1 468.12cM,各连锁群的长度变幅为114.24~189.34cM,标记间平均图距为3.38cM。【结论】成功构建了一张密度较高的高丹草分子遗传连锁图谱。【Objective】In order to identify the QTLs of hydrocyanic acid,seed yield and other important traits of Sorghum-Sudangrass,agenetic linkage map was constructed with AFLP marker to lay a foundation for marker-assisted selection breeding.【Method】Screening suitable AFLP primers combinations and PCR amplification of these primers by using AFLP molecular marker technique,Join Map 3.0software were applied to construct a genetic linkage map of Sorghum-Sudangrass for amplification data,with 305 individuals of an F2 population from a cross of Sorghum bicolor×Red hull Sudangrass as a mapping population.【Result】A total of 25 suitable primer combinations with high polymorphism,good repeatability and clear bands were screened from 113 AFLP primer combinations,and used to amplified 444 AFLP markers from genomic DNA of 305 individuals of the mapping population;the constructed genetic linkage map contained 10 linkage groups,and covered 1 468.12 cM of the total genome length.The length of each linkage group ranged from 114.24 to 189.34 cM,and the average distance between markers was 3.38 cM.【Conclusion】It is a genetic linkage map of Sorghum-Sudangrass with a high marker density.

关 键 词：高丹草 AFLP分子标记 遗传连锁图谱 

分 类 号：S544.9[农业科学—作物学]