芪水煎对糖尿病肾病小鼠肝、肾组织细胞外基质调节因子表达的影响  

Effects of Qishui decoction on the expression of extracellular matrix regulatory factors in liver and kidney of diabetic nephropathy mice

作　　者：刘晔[1] 车念聪[1] 杜宇琼[1] 王金光[1] 李文新[1] 

机构地区：[1]首都医科大学中医药学院中医临床基础学系,北京硕士研究生100069

出　　处：《环球中医药》2017年第10期1170-1174,共5页Global Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)(2012CB518602)

摘　　要：目的基于肝肾同源理论,观察糖尿病肾病(kk-ay)小鼠后期谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶含量(aspartate transaminase,AST)变化以及芪水煎对肝、肾基质金属蛋白酶-9(Matrix metalloe proteinas-9,MMP-9)及基质金属蛋白酶组织抑制因子-1(tissue inhibitor of metalloproteinase-1,TIMP-1)表达的影响。方法采用kk-ay小鼠高糖高脂饮食4周建立糖尿病肾病模型后,随机分为模型组、黄芪水组、通心络组,设立C57BL/6J小鼠作为对照组。予芪水煎及通心络干预12周后,监测各组小鼠血ALT、AST,采用Western Blot、Realtime-PCR检测肝、肾组织MMP-9和TIMP-1蛋白含量及m RNA表达水平。结果与模型组比较,芪水煎组AST显著降低(P<0.01)、ALT呈下降趋势(P>0.05),肝MMP-9蛋白及m RNA表达显著升高(P<0.01),TIMP-1蛋白显著降低(P<0.01),m RNA表达降低(P<0.05),肾MMP-9蛋白及m RNA表达升高(P<0.05),TIMP-1蛋白及m RNA表达降低(P<0.05)。结论 kk-ay小鼠后期会出现肝损伤,芪水煎对kk-ay小鼠肝、肾损伤具有一定的保护作用。Objective To investigate the content changes of alanine aminotransferase （ALT） and aspartate aminotransferase （AST） of diabetic nephropathy （kk-ay） mice in later stage, and to investigate the influence of Qishui decoction on the expression of MMP-9, TIMP-1 in the hepatic and nephritic tissues based on the theory of ＆quot;homogeny of liver and kidney＆quot;. Methods The diabetic nephropathy model was established by kk-ay mice with high glucose and high fat diet for 4 weeks. The mice were randomly divided into model group, Qishui group and Tongxinluo group, and C57BL/ 6J mice were used as control group. After 12 weeks of drug intervention, the level of ALT and AST was detected, and the protein and mRNA expressions of MMP-9 and TIMP-1 in liver and kidney were detected by Western Blot and realtime-PCR. Results Compared with the model group, the level of AST in Qishui group was significantly decreased （P〈0. 01）, while the level of ALT was decreased （ P 〉 0. 05）. The protein and mRNA expression of MMP-9 in liver was significantly increased （ P 〈 0. 01）, while the protein expression of TIMP-1 was significantly decreased （ P 〈 0. 01）, and mRNA was decreased （ P 〈 0. 05）. The protein and mRNA expression of MMP-9 in kidney was increased （ P〈0. 05）, while the protein and mRNA expression of TIMP-1 was decreased （P〈0. 05）. Conclusion kk-ay mice have liver injury in the later stage of diabetic nephropathy, and Qishui decoction has protective effects on liver and kidney injury in kk-ay mice.

关 键 词：黄芪 水红花子 肝肾同源 肝损伤 肾基质金属蛋白酶-9 基质金属蛋白酶组织抑制因子-1 

分 类 号：R285.5[医药卫生—中药学]