复发性自然流产患者T细胞中T细胞免疫球蛋白和免疫受体酪氨酸抑制基序的表达  

Expression of T cell immunoglobulin and immunoreceptor tyrosine-based inhibitory motif domain on T cells in patients with recurrent spontaneous abortion

作　　者：陈颖[1] 李翠 应春妹 

机构地区：[1]上海集爱遗传与不育诊疗中心,上海200011 [2]复旦大学附属妇产科医院检验科,上海200011

出　　处：《诊断学理论与实践》2017年第3期273-276,共4页Journal of Diagnostics Concepts & Practice

摘　　要：目的:探讨复发性自然流产(recurrent spontaneous abortion,RSA)患者T细胞中T细胞免疫球蛋白和免疫受体酪氨酸抑制基序(T cell immunoglobulin and immunoreceptor tyrosine-based inhibitory motif domain,TIGIT)的表达情况,为其可能作为治疗靶点提供理论依据。方法:采集21例正常早孕者及23例RSA患者的抗凝外周血,分别检测其CD3^+T细胞表面分子TIGIT的百分比及细胞因子IL-10和IFN-γ的表达情况。结果:RSA组患者TIGIT+细胞所占CD3^+T细胞的百分比为(41.52±7.17)%,高于早孕组中的该百分比[(36.09±4.03)%],差异有统计学意义(P<0.05);RSA组TIGIT+CD3^+T细胞中IL-10阳性细胞所占百分比分别为(11.68±5.90)%,低于早孕组TIGIT+CD3^+T细胞中IL-10阳性细胞所占百分比[(16.33±6.65)%],差异有统计学意义(P<0.05);而CD3^+T细胞中IFN-γ的表达在2组间差异无统计学意义。结论:负性共刺激分子TIGIT在RSA患者中异常表达,可能参与了母胎免疫的调控,与RSA的发生相关。Objective： To investigate the expression of TIGIT （T cell immunoglobulin and immunoreceptor tyrosine- based inhibitory motif domain） on peripheral T cells in patients with recurrent spontaneous abortion （RSA）. Methods： Proportion of TIGIT＋ T ceils and the expression of IFN-γ and IL-10 of TIGIT＋ T cells were detected in 21 normal pregnant women and 23 RSA patients. Results： Compared with normal pregnant women, the proportion of TIGIT^＋ T cells were detected in RSA patients was significantly higher [（41.52±7.17）% vs （36.09±4.03）%）] （P〈0.05）, and the IL-10 expression of TIGIT＋ T cells in RSA group were lower than normal pregnant group [（11.68±5.90）% vs （16.33±6.65）% ]（P〈0.05）. The expression of IFN-γ in the two groups had no significant difference. Conclusions： The abnormal expression of TIGIT may be involved in the regulation of maternal-fetal immune tolerance and is related with the occurrence of RSA.

关 键 词：复发性自然流产 T细胞免疫球蛋白和免疫受体酪氨酸抑制基序 机制 

分 类 号：R541.7[医药卫生—心血管疾病]