玻璃化冻融卵裂期胚胎体外培养时间对妊娠结局的影响  

Study on the impact of post-thawed culture of vitrified embryos on pregnancy outcomes

作　　者：王晟[1] 郐艳荣[1] 张凯[1] 曾诚[1] 王玲[1] 徐阳[1] 

机构地区：[1]北京大学第一医院妇产科,北京100034

出　　处：《中国性科学》2017年第10期128-131,共4页Chinese Journal of Human Sexuality

摘　　要：目的:研究人卵裂期胚胎玻璃化冷冻复苏后的体外培养时间对妊娠结局的影响。方法:回顾性分析2016年1月1日至2016年11月30日在我中心进行冻融胚胎移植(frozen-thawed embryo transfer,FET)治疗的患者492个周期。根据胚胎解冻后是否过夜培养分为两组,A组为解冻后过夜培养组,共270个周期;B组为解冻当日移植组,共222个周期。根据过夜培养后胚胎是否生长,将A组继续分为三个亚组,A1组:全部胚胎均生长,共161例;A2组:部分胚胎生长,共89例;A3组:无胚胎生长,共20例。比较各组患者年龄、内膜厚度、移植胚胎数、胚胎复苏率、临床妊娠率及胚胎着床率。结果:A组与B组在患者年龄、内膜厚度、胚胎复苏率、移植胚胎个数、临床妊娠率和胚胎着床率等方面均无统计学差异(P>0.05)。A1、A2、A3三组之间在患者年龄、内膜厚度、移植胚胎数及临床妊娠率等方面均无统计学差异(P>0.05),但是A1组的胚胎复苏率(92.99%)显著高于A3组(83.64%)(P<0.05),A1组的胚胎着床率(28.12%)分别显著高于A2组(19.53%)和A3组(13.04%)(P<0.05)。结论:胚胎复苏后是否过夜培养并不影响妊娠结局,但是过夜培养后继续生长的胚胎具有更高的着床率。Objectives： To study the impact of post-thawed culture of vitrified embryos on pregnancy outcomes. Methods： A total of 492 frozen-thawed embryo transfer（ FET） cycles in the reproductive center of Peking University First Hospital from January 1,2016 to November 30,2016 were analyzed retrospectively. Depending on the post-thawed culture time,they were divided into two groups,group A（ cultured overnight before transfer,n =270） and group B（ cultured 1 ~ 4 h before transfer,n = 222）. According to the embryos development after the post-thawed culture overnight,group A was subdivided into three groups： all the embryos developed（ as group A1,n= 161）,some of the embryos developed（ as group A2,n = 89） and none of the embryos developed（ as group A3,n = 20）. Age,endometrium thickness,number of embryo transferred,embryo survival rate,clinical pregnancy rate and implantation rate of these groups were compared. Results： There were no significant difference between group A and group B in age,endometrium thickness,number of embryo transferred,embryo survival rate,clinical pregnancy rate,and implantation rate（ P 0. 05）. Also,there were no significant difference in age,endometrium thickness,number of embryo transferred and clinical pregnancy rate among group A1,group A2 and group A3（ P 0. 05）.The embryo survival rate of group A1（92. 99%） was significantly higher than that of group A3（83. 64%）（ P 0. 05）. The clinical implantation rate of group A1（ 28. 12%） was significantly higher than that of group A2（19. 53%） and group A3（13. 04%）,the differences were statistically significant（ P 0. 05）. Conclusion：There is no impact of embryos culture time after vitrified thawed on pregnancy outcomes. However,embryos developed overnight has higher implantation rate.

关 键 词：玻璃化冷冻 冻融胚胎 体外培养 妊娠结局 

分 类 号：R711.6[医药卫生—妇产科学]