BDNF联合chABC治疗对脊髓损伤大鼠的神经保护作用研究  

Effect of combined treatment of brain-derived neurotrophic factor with chondroitin ABC on neurological function and glial scar in spinal cord injury rats

作　　者：李晓彬[1] 刘涛[1] 周俊锋[1] 范磊[1] Li Xiaobin Liu Tao Zhou Junfeng Fan Lei(Department of Emergency Traumatology, People＇s Hospital of Henan Province, Zhengzhou 450003, Chin)

机构地区：[1]河南省人民医院急诊创伤外科,郑州450003

出　　处：《中华神经医学杂志》2017年第10期1028-1033,共6页Chinese Journal of Neuromedicine

基　　金：河南省基础与前沿技术研究计划（122300410195）

摘　　要：目的探讨脑源性神经营养因子（BDNF）联合硫酸软骨素酶ABC（chABC）治疗对脊髓损伤大鼠脊髓损伤局部胶质瘢痕形成的影响及其神经保护作用。方法健康雌性SD大鼠80只按随机数字表法分为假手术组、生理盐水治疗组、BDNF治疗组、chABC治疗组、BDNF联合chABC治疗组，每组16只。后4组大鼠制作脊髓损伤模型，术后即刻分别于损伤中心处注射10μL生理盐水、20μL BDNF、6μL chABC、6μL chABC＋20μL BDNF。假手术组大鼠不做任何治疗。伤后1d、7d、14d、28d，每组取4只大鼠，采用HE染色、Nissl染色观察脊髓损伤处胶质瘢痕形成情况，采用免疫组化染色检测脊髓损伤处胶质纤维酸性蛋白（GFAP）的表达。结果伤后1d，HE染色、Nissl染色显示后4组大鼠脊髓损伤中心灰质出血明显，炎症细胞少量聚集，尼氏体开始由斑片状分解为细尘粒状；伤后7d，各治疗组的尼氏体分解成细尘状颗粒，神经元死亡较多，星形胶质细胞活化增生，炎症细胞浸润；伤后14、28d，瘢痕组织及空洞形成，星形胶质细胞继续增生，尼氏体逐渐由细尘状恢复到虎斑状，着色神经元数量逐渐恢复。后3组尼氏体的恢复、神经元数量均多于生理盐水治疗组，其中BDNF联合chABC治疗组恢复最优。伤后1d，后4组大鼠脊髓损伤处开始出现GFAP的阳性表达，随着时间的延长逐渐增多，伤后14d达高峰，28d时稍有下降，任2个时间点间GFAP阳性表达比较差异均有统计学意（P〈0．05）。伤后7d，BDNF联合chABC治疗组GFAP染色阳性面积低于生理盐水治疗组；伤后14、28d，BDNF、chABC单独及联合治疗组GFAP染色阳性面积均低于生理盐水治疗组：伤后28d．BDNF联合chABC治疗组GFAP染色阳性面积低于生理盐水治疗组和BDNF、chABC治疗组，差异均有统计学意义（P〈0．05）。结论BDNF联合chABC治疗对脊髓损伤具有神经保护和神经营养作用，可以减Objective To evaluate the effect of brain-derived neurotrophic factor （BDNF） combined with chondroitin ABC （chABC） on neurological function recovery and partial glial scar formation in rats with spinal cord injury （SCI）. Methods Eighty SD rats were randomly divided into sham-operated group, saline treatment group, BDNF treatment group, chABC treatment group and BDNF combined with chABC treatment group （n=16）; rats in the later 4 groups were established SCI rat models; immediately after SC1, 10 μL saline, 20 μL BDNF, 6μL chABC and 6 μL chABC＋20 μL BDNF were given, and rats in the sham-operated group did not give any treatment. One, 7, 14 and 28 d after SCI, HE and Nissl stainings were employed to observe the glial scar formation; immunohistochemical staining was used to detect the glial fibrillary acidic protein （GFAP） expression. Results One d after SCI, HE staining and Nissl staining showed that obvious hemorrhage in the grey matter and aggregated inflammatory cells were observed in the SCI center, and Nissl substance began to break up into dust particles from speckle; 7 d after SCI, Nissl substance broke up into dust particles from speckle, and died neurons, activated astrocytes and infiltrated inflammatory cells were observed; 14 and 28 d after SCI, glial scars were formed, activated astrocytes were noted, Nissl substance recovered from dust particles into maculosus, and number of colored neurons increased; the recovery of Nissl substance and number of neurons in the BDNF treatment group, chABC treatment group and BDNF combined with chABC treatment group were better than those in the saline treatment group, and BDNF combined with chABC treatment group enjoyed the best recovery. One d after SCI, GFAP positive expression began in the rats of the later 4 groups, and peaked 14 d after SCI; 7 d after SCI, GFAP positive labeling areas in the BDNF combined with chABC treatment group were smaller than those in the saline treatment group; 14 and 28 d after SCI, GFAP positive labeling areas in

关 键 词：脊髓损伤 硫酸软骨索酶ABC 脑源性神经营养因子 胶质瘢痕 

分 类 号：R651.2[医药卫生—外科学]