巨大芽孢杆菌LY02对黑麦草修复重金属污染土壤的影响  

Effect of Bacillus Megaterum LY02 on Phytoremediation of Heavy Metal from Contaminated Soil by Lolium Perenne L.

作　　者：赵树民 李晓东 虞方伯 鲍雅莉 沈颖 张媚[1] 林马水 王冰璇 林海萍[1] ZHAO Shumin LI Xiaodong YU Fangbo BAO Yali SHEN Ying ZHANG Mei LIN Mashui WANG Bingxuan LIN Haiping(National Joint Engineering Laboratory of Biopesticide Preparation ,School of Forestry and Biotechnology, Zhejiang Agricultura! and Forestry University, Hangzhou 311300 Lishui Forestry Bureau of Zhejiang , Lishui, Zhej iang 323000 Key Laboratory o f Soil Contamination Biorernediation of Zhejiang Province, Hangzhou 311300)

机构地区：[1]浙江农林大学生物农药高效制备技术国家地方联合工程实验室,杭州311300 [2]浙江省丽水市林业局开发区分局,浙江丽水323000 [3]浙江省土壤污染生物修复重点实验室,杭州311300

出　　处：《水土保持学报》2017年第5期340-344,共5页Journal of Soil and Water Conservation

基　　金：浙江省科技厅公益技术研究农业项目(2015C32078);浙江省重点创新团队项目(2013TD12)

摘　　要：采用盆栽试验方法,研究了接种巨大芽孢杆菌LY02对黑麦草修复Cd、Cu污染土壤以及二者复合污染土壤效果的影响。结果表明:在Cd、Cu污染土壤及复合污染土壤中接种巨大芽孢杆菌LY02,可显著提高黑麦草地上部生物量,增幅达65.0%~108.3%;促进了黑麦草在3种污染土壤中对重金属的吸收,其中对Cd污染土壤中黑麦草吸收Cd的影响最为显著,地上部Cd吸收量较对照组增加了45.8%(P<0.05);3种污染土壤中黑麦草根际土壤有效磷含量显著升高,较对照组分别增加了18.2%,26.7%,16.2%;黑麦草根际土壤中有效态Fe含量显著提高,Cu单一污染土壤中增幅最大,达到152.5%;3种污染土壤中有效态重金属含量升高,在Cu污染土壤中,有效态Cu增幅达到49.7%(P<0.05)。综上所述,巨大芽孢杆菌LY02通过增加污染土壤中生物可利用态P和Fe,促进黑麦草生长;通过提高有效态Cd和Cu的含量,增加黑麦草对其吸收,从而提高了黑麦草对Cu、Cd污染土壤的修复效率。Pot experiments were carried out to evaluate the influence of Bacillus megaterum LY02 inoculation on phytoremediation of Cd,Cu and Cd-Cu contaminated soil by Lolium perenne L.The results showed that inoculation B.megaterum LY02 in Cd,Cu and Cd-Cu contaminated soil significantly increased the aboveground biomass of Lolium perenne L.by 65% ~108.3% .The strain promoted phytoextration of heavy metal by Lolium perenne L.in different kinds of contaminated soil, and Cd extraction was increased significantly by 45.8% compared with the control.Compared with the control,B.megaterum siginificantly enhanced the available phosphorus in three kinds of contaminated soil by 18.2% ,26.7% and 16.2% ,respectively.The available Fe was increased after inoculation,especially in Cu-contaminated soil,the increase was up to 152.5% .Furthermore,the content of available Cd and Cu in the rhizosphere were also enhanced in the three kinds of contaminated soil,the available Cu was increased by 49.7% in the Cu-contaminated soil.Above all,inoculation B.megaterum LY02 in Lolium perenne L.can improve the content of available P and Fe in the contaminated soil, and enhance the content of available Cd and Cu,which are beneficial for the improvement of remediation efficiency of Lolium perenne L..

关 键 词：巨大芽孢杆菌 黑麦草 植物修复 CD CU 

分 类 号：X53[环境科学与工程—环境工程]