中华绒螯蟹免疫蛋白互作网络提取及分析  

Extraction and analysis of Eriocheir sinensis immune protein-protein interaction network

作　　者：王彬[1,2,3] 宁黔冀 王倩[2,3] 彭玮[2,3] 郝彤 孙金生[2,3] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007 [2]天津师范大学生命科学学院,天津300387 [3]天津师范大学天津市动植物抗性重点实验室,天津300387

出　　处：《天津师范大学学报（自然科学版）》2017年第4期40-45,共6页Journal of Tianjin Normal University：Natural Science Edition

基　　金：国家高技术研究发展计划(863计划)资助项目(2012AA10A401;2012AA092205);国家重点基础研究发展计划(973计划)资助项目(2012CB114405);国家自然科学基金资助项目(21106095);国家科技支撑计划资助项目(2011BAD13B07;2011BAD13B04);天津市应用基础与前沿技术研究计划资助项目(15JCYBJC30700);天津市"三年引进千名高层次人才"计划资助项目(5KQM110003);天津市"131"创新型人才培养工程资助项目(ZX110170);天津师范大学青年教师学术创新推进计划资助项目(52XC1403)

摘　　要：为了研究中华绒螯蟹的免疫蛋白作用机制,在已构建的中华绒螯蟹蛋白互作网络基础上,采用邻接节点注释方法对其全局网络进行细胞组分注释,为网络的903个未定位蛋白中的830个添加了GO注释,占未定位蛋白的91.9%.从中华绒螯蟹蛋白互作网络中提取了包含142个蛋白和158条蛋白互作关系的免疫互作网络,并且结合GO细胞组分注释信息,得到了其中62个免疫蛋白的细胞组分定位.通过对细胞定位的分析发现,中华绒螯蟹的免疫蛋白多位于膜结构、细胞核和细胞骨架中.To learn the active mechanism of immune proteins in , the neighbor-based annotation methodwas adopted to annotate the subcellular locations of the proteins in the previously reconstructed protein-protein interactionnetwork of global. GO annotations were added to 830 proteins among 903 unidentified proteins in the net-work, accounting for 91.9 of all the unidentified proteins. Simultaneously, the immune protein-protein interaction networkwas extracted, which contains 142 proteins and 158 protein interactions, from the global protein-protein interaction network.Furthermore, the subcellular locations of 62 immune proteins were obtained combined with GO cellular component annota-tion information. The analysis of subcellular localization revealed that the immune proteins were mostly located in the mem-brane structure, nucleus and cytoskeleton.

关 键 词：中华绒螯蟹 蛋白互作网络 邻接节点注释 免疫蛋白 

分 类 号：Q816[生物学—生物工程]