小花棘豆和苦马豆不同萃取相乙酰胆碱酯酶活性及机理探讨  

Acetylcholinesterase Activity and Mechanisms of Different Extracts from Oxytropis glabra DC. and Swainsonia salsula (Pall.) DC.

作　　者：王志雁 沈红玲[1] 孟庆艳[2] 张航航 贾旭[1] 刘文杰[1,2] 

机构地区：[1]塔里木大学生命科学学院 [2]新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室,新疆阿拉尔843300

出　　处：《天然产物研究与开发》2017年第10期1706-1711,共6页Natural Product Research and Development

基　　金：国家自然科学基金(21365018);新疆生产建设兵团科技攻关项目(2011AB030);新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室开放课题(BRYR1404)

摘　　要：本文研究了小花棘豆及苦马豆对乙酰胆碱酯酶(AChE)的抑制活性及抑制动力学,为进一步开展植物中乙酰胆碱酯酶抑制剂奠定了基础。采用超声辅助溶剂提取、有机溶剂梯度萃取等方法得到各相提取物,运用改良的Ellman比色法对小花棘豆和苦马豆进行了AChE抑制活性和动力学研究。结果表明,小花棘豆根、叶以及苦马豆叶的氯仿相萃取物具有较高的AChE抑制活性,其IC_(50)值分别为0.67、1.69 mg/mL和0.17 mg/mL,而其他萃取相表现出较弱的AChE抑制活性。此外,小花棘豆和苦马豆叶氯仿相的抑制类型均为可逆的混合型抑制。In order to evaluate the activity and potential application of acetylcholinesterase inhibitors from Oxytropis glabra DC.and Swainsonia salsula （Pall.） DC.,the inhibition mechanism and kinetics were investigated in this study.Each extract was prepared using different polarity solvents associated with ultrasonic assisted extraction.In addition,improved Ellmans＇ colorimetric method was employed to exploit the acetylcholinesterase inhibition activity and kinetics.Results showed that the chloroform extracts of both root and leaf of O.glabra and the leaf of S.salsula demonstrated higher AChE inhibitory activities over other extracts with the IC50 values of 0.67 mg/mL,1.69 mg/mL and 0.17 mg/mL,respectively.The inhibition mechanism of chloroform extracts from leaf of these two plants are both mixed type reversible inhibition.

关 键 词：萃取物 乙酰胆碱酯酶 抑制机理 动力学 

分 类 号：Q553[生物学—生物化学]