碳酸酐酶在Fe_3O_4表面固定及性能分析研究  

Immobilization and Characterization of Carbonic Anhydrase on Ferriferrous Oxide

作　　者：李娟[1] 周心澄[1] 张琳[1] 杨林军[1,2] 

机构地区：[1]东南大学能源热转换及其过程测控教育部重点实验室,南京210096 [2]中国矿业大学江苏省煤基CO_2捕集与地质封存重点实验室,徐州221008

出　　处：《科学技术与工程》2017年第25期151-157,共7页Science Technology and Engineering

基　　金：国家自然科学基金(51176034);高等学校博士学科点专项科研基金(20130092110005);江苏省煤基CO2捕集与地质储存重点实验室(2016年)开放基金(2016A05);东南大学优秀博士学位论文基金(YBJJ1508)资助

摘　　要：首先将羧基官能团引入纳米级Fe_3O_4表面,并用交联剂戊二醛将碳酸酐酶(carbonic anhydrase,CA)固定到Fe_3O_4上,即得到固定化酶。采用比表面积(BET)、热重分析(TG)、傅里叶红外(FTIR)、X射线衍射(XRD),扫面电镜(SEM)等手段对载体材料和固定化酶的物理化学性质进行表征对比。以对硝基苯酚乙酸酯(p-NPA)为底物,采用紫外分光光度计测定酶活。结果表明,制备的载体材料具有较大的比表面积,表面羧基化后,载体共价结合后的有机物含量达到11.24%;载体材料表面不存在C=C。采用戊二醛进行酶的固定化试验时,固定化酶活性最大可达到68.26%;且固定化酶比游离态酶具有更高的操作稳定性和贮藏稳定性。本方法有望将固定化酶应用在连续式填料塔反应器中以提高其低浓度CO_2的去除效率。Firstly carboxyl groups were introduced onto the surface of nano ferroferric oxide（Fe_3O_4）,and then the carbonic anhydrase（ CA） was immobilized onto the carrier by crosslinking reaction with glutaraldehyde,which meant the immbilized CA was obtained. The physical and chemical properties of the cairier and immobilized enzyme were characterized and compared by specific surface area（ BET）,thermal gravimetric analysis（ TG）,fourier transform infrared spectroscopy（ FTIR）,X-ray diffraction（ XRD） and scanning electron microscope（ SEM）. Pnitrophenol acetate（ p-NPA） was substrate to determine the activity,operation stability and storage stability of the immobilized CA by the ultraviolet spectrophotometer（ UV）. The results show that the carrier has a larger surface area; after carboxyl-functionalized,the organic content of the carrier can reach 11. 24%; there was no C=C on the surface of the carrier. In the immobilization experiment using glutaraldehyde,the activity of immobilized enzyme could reach the maximum value： 68. 26%. Besides,the immobilized CA had higher the operation stability and storage stability than that ofthe free CA. The method is expected to be applied in the continuous packed column reactor to improve the removal efficiency of CO_2 under the low concentration.

关 键 词：碳酸酐酶 FE3O4 羧基化 固定化 戊二醛 

分 类 号：X511[环境科学与工程—环境工程]