医用纯Mg表面多种复合处理膜层的组织结构和体内外性能  

The Microstructure and in Vivo and in Vitro Property of Multi-Component Composite Films on the Biomedical Pure Magnesium Surface

作　　者：李慕勤[1] 姚海涛[1] 魏方红[1] 刘明达[1] 王赞[1] 彭书浩 

机构地区：[1]佳木斯大学黑龙江省高校生物医学材料重点实验室,佳木斯154007

出　　处：《金属学报》2017年第10期1337-1346,共10页Acta Metallurgica Sinica

基　　金：国家自然科学基金面上项目No.31370979~~

摘　　要：为了提高医用纯Mg的耐蚀性和生物活性,采用超声微弧氧化(UMAO)、碱处理(OH)、硅烷转化膜处理(SCA)和自组装丝素蛋白(SF)多种处理方法,在Mg表面形成多元膜层(UMAO-OH-SCA-SF),通过SEM、红外光谱、电化学测试、细胞实验和动物植入实验,研究膜层表面形貌、组织结构、耐腐蚀性能、细胞活性和体内骨生长。结果表明,超声微弧氧化膜层以MgO为主相,碱羟基利于与硅烷偶联形成Si-O-Mg膜,再自组装丝素,随着浸泡丝素蛋白时间增加,丝素蛋白的结构由无规卷曲向b-折叠转变,多元复合膜层提高了自腐蚀电位,自腐蚀电流密度降低了2个数量级。多元复合膜层更能促进成骨细胞的增殖、黏附与分化,具有良好的早期骨整合能力和在纯Mg降解过程中控制Mg^(2+)溶出能力,其中UMAO-OH-SCA-SF/1.5h最佳。Entering into 21 Century,the degradable Mg and Mg alloy become research focus for the development of Internal fixation material from inert to active metal. The polybasic coating（UMAO-OHSCA-SF） was prepared by ultrasound micro-arc oxidation（UMAO）, alkali treatment（OH）, treatment of silicohydride conversion coating（SCA） and the treatment of self-assembly silk fibroin on the surface of magnesium, which can improve the corrosion resistance and bioactivity of pure magnesium. The surface topography, structure, corrosion resistance, cell activity and bone growth in vivo of the coating were studied by SEM, IR spectra, electrochemical measurement, vitro experiment and implant test. The results show that the main phase of coating is Mg O. The alkali treatment is beneficial to forming Si-O-Mg film by silicohydride coupling. With the self-assembly silk fibroin time increasing, the silk fibroin structure changes from random coil to b-fold. The polybasic coating self-corrosion is improved and self-corrosion current is reduced by two orders of magnitude. Compared to substrate, the polybasic coating has better proliferation, adherent and differentiation of osteoblast. It has a better bone integration capacity in the bone healing early stage, and which can control magnesium ion dissolving. The UMAO-OH-SCA-SF/1.5 h coatinghas the best property.

关 键 词：纯MG 超声微弧氧化 硅烷 丝素蛋白 耐蚀性 生物活性 

分 类 号：R318.08[医药卫生—生物医学工程]