CD4基因的DNA甲基化修饰在家畜抗病育种中的应用  

DNA Methylation Modification of CD4 Gene and Its Application in Livestock Diseases-Resistant Breeding

作　　者：王晓铄[1,2] 赵会敏 唐天 李凯 钱旭 张雪 图雅 郑云胜[1] 王怀栋[1] 俞英[2] WANG Xiao-shuo ZHAO Hui-min TANG Tian LI Kai QIAN Xu ZHANG Xue TU Ya ZHENG Yun-sheng WANG Huai-dong YU Ying(Vocational and Technical College of Inner Mongolia Agricultural University, Baotou 014109, China College of Animal Science and Technology, China Agricultural University, Beijing 100193, China)

机构地区：[1]内蒙古农业大学职业技术学院,包头014109 [2]中国农业大学动物科技学院,北京100193

出　　处：《畜牧兽医学报》2017年第10期1796-1806,共11页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：内蒙古自然科学基金(2016BS0305);国家自然科学基金(31272420;31572361);北京市奶牛产业创新团队项目(BAIC06-2017)

摘　　要：CD4分子表达于成熟的CD4^+T细胞表面,能够指导T细胞的正常分化与成熟,识别抗原、介导免疫应答并参与免疫调节。DNA甲基化对T细胞亚群的分化调控等方面具有重要作用,特别是CD4基因的DNA甲基化显著影响CD4+T细胞数量和功能,能够在一定程度上改变家畜的免疫应答能力或抗病性能,以对抗或适应外界环境改变。目前,已发现猪和奶牛的CD4基因DNA甲基化可调控宿主细胞的免疫反应,但在羊和马上鲜有报道。为深入了解家畜复杂疾病形成的分子基础,以及为利用分子标记有效选育抗病个体提供新的思路,本文主要就CD4+T淋巴细胞分化过程中,白细胞分化抗原CD4基因的DNA甲基化修饰对免疫反应的调控机制,以及其在家畜抗病育种中的应用前景进行综述和展望。CD4 molecular is mainly expressed on mature CD4^＋T cells surface. It can direct T cells normal differentiation and maturation, recognize antigen, mediate immune response and involve in immune regulation. DNA methylation modification is critical to regulating differentiation of T cell subsets. Especially, the DNA methylation of CD4 gene can significantly affect the amount and function of CD4^＋T cells, influence the ability of immune response and disease resistance of livestock, then against or adapt environmental changes. Up to date, studies have reported that DNA methylation of porcine and bovine CD4 gene can regulate immune responses of the host cells, while that of sheep and horse are rarely reported. To understand the molecular basis of complex diseases and provide novel breeding strategies against livestock diseases via molecular marker assistant selection methods, the progress on regulation mechanism of DNA methylation of livestock CD4 gene, and its effects on immune responses during CD4^＋T cells differentiation, as well its possible application in disease resistance breeding of livestock were reviewed and discussed in this paper.

关 键 词：家畜 CD4基因 DNA甲基化 抗病育种 

分 类 号：Q953[生物学—动物学] S813.1[农业科学—畜牧学]