陕西省犊牛安氏隐孢子虫多位点序列分型研究  

Multilocus Sequence Typing of Cryptosporidium andersoni from Calves in Shaanxi Province

作　　者：任冠静 王雪婷[1] 张会军 宋军科[1] 赵光辉[1] REN Guan-jing WANG Xue-ting ZHANG Hui-jun SONG Jun-ke ZHAO Guang-hui(College of Veterinary Medicine, Northwest A＆ F University, Yangling 712100, China)

机构地区：[1]西北农林科技大学动物医学院,杨凌712100

出　　处：《畜牧兽医学报》2017年第10期1969-1975,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家重点研发计划(2017YFD0501300);国家自然科学基金面上项目(31572509);陕西省科学技术研究发展规划资助项目(2016NY-113);中国博士后面上项目(2016M592848);陕西省博士后科研项目资助特别资助

摘　　要：为了对陕西省犊牛安氏隐孢子虫(Cryptosporidium andersoni)进行多位点序列分型(multilocus sequence typing,MLST),本研究基于4个基因位点对陕西省4个地区的36份犊牛(0~6月龄)C.andersoni阳性粪便样品进行PCR检测和序列分析。结果显示,15份C.andersoni分离株在CM-MS1、CM-MS2、CM-MS3、CM-MS16 4个基因位点均被成功分型,形成A4、A4、A2、A1,A4、A4、A4、A1,A5、A4、A4、A1和A1、A4、A4、A1共4个MLST亚型。其中A4、A4、A4、A1广泛分布于各个养殖场与不同品种犊牛中,为该地区犊牛C.andersoni的优势亚型。该研究结果表明,陕西地区犊牛C.andersoni存在遗传多样性,具有多种MLST亚型。To study the multilocus sequence types of Cryptosporidium andersoni from calves in Shaanxi Province, 36 fecal samples positive for C. andersoni were collected from 0-6 months calves in four regions of Shaanxi province and examined by the polymerase chain reaction （PCR）. Sequence analysis was based on four genetic loci, namely CM-MS1, CM-MS2, CM-MS3 and CM- MS16. The results showed that a total of 15 C. andersoni isolates were successfully genotyped in all four loci and divided into 4 subtypes of MLST, namely A4, A4, A2, A1; A4, A4, A4, A1; AS, A4, A4, AI; A1, A4, A4, A1. Among them, the MLST （A4, A4, A4, A1）, as the dominant subtype, was widely distributed in various farms and different breeds of calves. These findings indicated that genetic variation was presented in C. andersoni of calves in Shaanxi province and various MLST subtypes were found in these calves.

关 键 词：安氏隐孢子虫 多位点序列分型 犊牛 陕西 

分 类 号：S852.723[农业科学—基础兽医学]