新疆库蠓源性盖塔病毒的分离与鉴定  

Isolation and Identification of Getah Virus fromCulicoidesin Xinjiang

作　　者：刘帅[1,2] 加帕尔.哈斯木 薛新梅 丁梦玥 马晓菁[1] 叶锋[1] 马俊杰[1] 易新萍[1] 谷文喜[1] 钟旗[1] LIU Shuai Jiapaer HASIMU XUE Xin-mei DING Meng-yue MA Xiao-jing YE Feng MA Jun-jie YI Xin-ping GU Wen xi ZHONG Qi(Institute of Veterinary Medicine, Xinjiang Academy of Animal Science, Urumqi 830000, China College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China Yuli Animal Husbandry and Veterinary Station, Yuli 841500, China Animal Eppidemic Control and Diagnosis Center, Bayingol Mongolian Autonomous Prefecture, Korla 841000, China)

机构地区：[1]新疆畜牧科学院兽医研究所,乌鲁木齐830000 [2]新疆农业大学动物医学学院,乌鲁木齐830052 [3]新疆尉犁县畜牧兽医工作站,尉犁841500 [4]新疆巴音郭楞蒙古自治州动物疾病控制与诊断中心,库尔勒841000

出　　处：《畜牧兽医学报》2017年第10期1998-2004,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：公益性行业(农业)科研专项(201303035);自治区公益性科研院所基本科研业务经费资助项目(KY2017002)

摘　　要：为了解新疆尉犁县库蠓体内携带病毒情况,从该县采集18 000只库蠓,50只为一组研磨后分别接种于BHK-21、C6/36、Vero细胞,盲传5代,一组样品使BHK-21出现CPE,先用5-氟尿核苷药敏试验鉴定为RNA病毒,之后用虫媒RNA病毒引物扩增,鉴定为甲病毒,疑似盖塔病毒,最后用中和试验、免疫电镜观察和盖塔病毒C基因特异性引物PCR等方法鉴定分离毒株为盖塔病毒。本研究首次从新疆库蠓体内分离到盖塔病毒,该毒株与2005年日本分离株相似性高达98%,推测可能是由于引进日本带病种公畜或精液有关,提示在进出口畜牧贸易活动中,应加强盖塔病毒的检疫工作。In order to learn the situation of parasitic virus in Culicoides in Yuli County, Xinjiang, a total of 18 000 Culicoides were collected from Yuli County and divided into 360 groups, with 50 Culicoides in each group. Then the Culicoides were ground and inoculated into BHK-21, C6/36 and Vero cells, respectively. After 5 generations of blind passage, one group of Culicoides made CPE arise in BHK-21. Then the isolated strain was firstly identified to be RNA viruses by drug sensitivity test （5- floxuridine）. Secondly, the amplified primers were identified by arthropod-borne RNA viruses and the result showed that the strain belongs to alphaviruses and is suspected to be Getah viruses. Finally, the isolated strain was determined to be Getah viruses by neutralization test, immunoelectron microscopic observation, PCR amplification of Getah virus C gene-specific primer and other methods. In this study, Getah viruses were isolated from Xinjiang Culicoides for the first time and the virus strain presents a closest genetic relationship （98%） with the 2005 Japanese isolated strain, for which the reason might be the introduction of Japanese breeding sires with the disease or their seminal fluid, suggesting that in the import and export trade activities of animal husbandry, quarantine inspection on Getah virus should be strengthened.

关 键 词：新疆 库蠓 盖塔病毒 鉴定 

分 类 号：S852.659.6[农业科学—基础兽医学]