H_2S与Ca^(2+)互作提高黄瓜幼苗耐冷性  

Hydrogen Sulfide Interacts with Ca^(2+) to Enhance Chilling Tolerance of Cucumber Seedlings

作　　者：吴帼秀[1,2] 李丹丹 孙晨晨 孙胜楠[1] 刘丰娇[1] 毕焕改[1] 艾希珍[1] 

机构地区：[1]山东农业大学园艺科学与工程学院作物生物学国家重点实验室农业部黄淮地区园艺作物生物学与种质创制重点开放实验室,山东泰安271018 [2]河南农业大学园艺学院,郑州450002

出　　处：《中国生物化学与分子生物学报》2017年第10期1037-1046,共10页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金项目(No.31572170);山东省自然科学基金项目(No.ZR2015CM005)资助~~

摘　　要：硫化氢(hydrogen sulfide,H_2S)是一种新型气体信号分子,钙离子(calcium,Ca^(2+))为重要的第二信使,两者在调控植物生长发育及多种逆境胁迫中分别起着重要作用。然而,H_2S和Ca^(2+)信号在调控植物耐冷性中的作用关系并不十分清楚。本研究针对以上问题以‘津优35号’黄瓜为试材进行研究,结果表明,低温胁迫可诱导H_2S信号的产生,且这种信号可被外源Ca^(2+)增强;低温胁迫可诱导Ca^(2+)信号转导相关基因CaM、CIPK5的mRNA表达,且外源H_2S能够上调低温下CaM、CIPK5的表达量;进一步研究发现,外源H_2S和Ca^(2+)可显著增强植株的抗氧化能力,减少活性氧积累,从而降低低温胁迫对黄瓜幼苗的损伤,且加入Ca^(2+)螯合剂乙二醇二乙醚二胺四乙酸(ethylene glycol tetraacetic acid,EGTA)或钙调素拮抗剂氯丙嗪(chlorpromazine,CPZ)后,H_2S对黄瓜幼苗抗氧化能力的促进效应明显减弱,同样的,H_2S清除剂次牛磺酸(hypotaurine,HT)也会降低Ca^(2+)的作用。以上结果表明H_2S与Ca^(2+)信号间存在着复杂的联系,而且他们可以通过相互作用调控黄瓜幼苗的抗氧化系统,从而增强植株耐冷性。Hydrogen sulfide （H2S） is a new gasotransmitter and calcium （Ca2＋ ） is an important second messenger. Both play important roles in regulating plant growth development and adversity stresses, respectively. Here we used ＇ Jinyou 35 ＇ cucumber plants to study the relationship between H2S and Ca2＋ in regulating chilling resistance. The results showed that chilling stress could induce the generation of H2S signals, which could be enhanced by exogenous Ca2＋. Among the genes involved the Ca2＋ signal transduction pathway, the mRNA abundances of CaM and CIPK5 were stimulated in chilling stress and up-regulated by exogenous H2＋. Our studies also showed that exogenous H2＋ and Ca2＋ could remarkably strengthen the antioxidant ability and reduce accumulation of reactive oxygen species, thereby alleviatingthe damage of chilling stress to cucumber seedlings. Furthermore, the positive effect of H2＋ on the antioxidant ability was removed by adding the Ca2＋ chelating agent ethylene glycol tetraacetic acid （EGTA） or the calmodulin antagonist chlorpromazine （CPZ） , and the function of Ca2＋ was weakened by the H2S scavenger hypotaurine （HT）. We propose that there are complex connections between H2＋and Ca2＋ signaling, and that H2S and Ca2＋ may enhance chilling tolerance by adjusting the antioxidative system in cucumber seedlings.

关 键 词：黄瓜 硫化氢 钙 低温胁迫 抗氧化系统 

分 类 号：S642.2[农业科学—蔬菜学]