珍珠梅黄酮纳米粒调控STAT3抑制人肝癌HepG2细胞侵袭和转移作用的研究  

Study on TTF1-NP inhibits human hepatoma HepG2 cell migration and invasion through downregulation of STAT3

作　　者：幺雨含 张璇[1] 肖斌[1] 张学武[1] 

机构地区：[1]延边大学医学院,吉林延吉133002

出　　处：《时珍国医国药》2017年第9期2058-2060,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81460617);吉林省教育厅科技发展计划项目;延边大学大学生创新创业训练项目(No.ydbksky2017753)

摘　　要：目的探讨中药珍珠梅黄酮纳米粒(5,2’,4’-trihydroxy-6,7,5’-trimethoxy flavone nanoparticle,TTF1-NP)调控STAT3抑制人肝癌Hep G2细胞侵袭、转移的作用和分子机制。方法利用Western blot技术检测人肝癌HepG2细胞STAT3和p-STAT3蛋白表达,利用Transwell和细胞划痕技术检测人肝癌Hep G2细胞侵袭和转移能力,采用Western blot技术检测细胞侵袭迁移的相关蛋白MMP2和MMP9表达。结果 Western blot检测发现TTF1-NP可以抑制人肝癌Hep G2细胞STAT3的活化表达,通过抑制MMP2和MMP9表达抑制人肝癌Hep G2细胞侵袭和转移。结论 TTF1-NP通过抑制STAT3的活化抑制MMP2和MMP9蛋白表达抑制人肝癌Hep G2细胞侵袭和转移。Objective To investigate the mechanism of 5,2 ＇,4 ＇-trihydroxy-6,7,5 ＇-trimethoxy flavone nanoparticle（ TTF1-NP）-inhibited Hep G2 cell migration and invasion through down-regulation the activation of STAT3. Methods Western blot assay were used to investigate the effect of TTF1-NP on the activation of STAT3,transwell and scratch healing assay were used to test the invasion and migration effect of TTF1-NP on Hep G2 cells,western blot was used to detect the expression of migration-related proteins. Results Western blot assay showed that TTF1-NP markedly inhibited the activation of STAT3 and the expression of MMP2 and MMP9. Transwell and scratch healing assay showed that TTF1-NP markedly inhibited migration and invasion abilities. Conclusion TTF1-NP inhibited Hep G2 cell migration and invasion through the downregulation of MMP2 and MMP9 by inhibited the expression of STAT3.

关 键 词：珍珠梅黄酮纳米粒 肝癌 信号传导与转录激活因子3 侵袭 转移 

分 类 号：R285.5[医药卫生—中药学]