滤泡性辅助T细胞亚型与原因不明复发性流产的关系研究  

Relationship between subtypes of T follicular helper cells and unexplained recurrent spontaneous abortion

作　　者：栾晓蕊[1] 李卫平[1] 

机构地区：[1]上海交通大学医学院附属仁济医院妇产科,上海200127

出　　处：《上海交通大学学报（医学版）》2017年第10期1346-1349,共4页Journal of Shanghai Jiao tong University:Medical Science

摘　　要：目的·探讨原因不明复发性流产(URSA)的发生与滤泡性辅助T细胞(Tfh)亚型的关系。方法·以28例正常人工流产患者为对照组,28例染色体正常的URSA患者为流产组。分离2组患者外周血及子宫蜕膜组织中单个核细胞,用流式细胞术检测CXCR3^+CCR6^-、CXCR3^-CCR6^-及CXCR3^-CCR6^+3种Tfh细胞亚型水平及B细胞水平。结果·流产组蜕膜组织CXCR3^-CCR6^-Tfh细胞水平显著高于对照组(P=0.015),且与B细胞水平有相关性(R^2=0.779,P=0.025)。2组蜕膜组织中另外2种Tfh细胞亚型水平以及外周血中3种Tfh细胞亚型水平差异均无统计学意义(均P>0.05)。结论·早孕妇女蜕膜组织局部CXCR3-CCR6-Tfh细胞亚型数量增多可能与URSA发生有关。Objective · To discuss the relationship between unexplained recurrent spontaneous abortion （URSA） and T follicular helper （Tfh） cell subtypes. Methods · Twenty-eight normal early pregnancy women, who had undergone induced abortion, were taken as control, and 28 patients with URSA were enrolled in the abortion group. The mononuclear cells in peripheral blood and decidual tissues were separated in the two groups, and CXCR3^＋CCR6^- Tfh cells, CXCR3^-CCR6^- Tfh cells, CXCR3-CCR6＋ Tfh cells and B cells were tested by flow cytometry. Results · The decidual CXCR3CCR6^- Tfh cells significantly increased in the abortion group compared with control group （P=0.015）. And there was a strong association between the decidual CXCR3^-CCR6^- Tfh cells and B cells in URSA patients （R2=0.779, P=0.025）. Conclusion · The up-regulation of decidual CXCR3-CCR6- Tfh cells in early pregnancy women may be related with the occurrence of URSA.

关 键 词：原因不明复发性流产 滤泡性辅助T细胞 CXCR3 CCR6 蜕膜 流式细胞术 

分 类 号：R714.21[医药卫生—妇产科学]