米非司酮对人输卵管上皮纤毛摆动频率及雌、孕激素受体的影响  

Effect of mifepristone on ciliary beat frequency and estrogen and progesterone receptor expression levels in human fallopian tubes

作　　者：袁江静 赵伟红[1] 张朵 何晓英[1] 张健[1] 

机构地区：[1]上海交通大学医学院附属国际和平妇幼保健院妇科,上海200030 [2]上海交通大学医学院附属国际和平妇幼保健院中心实验室,上海200030

出　　处：《上海交通大学学报（医学版）》2017年第10期1402-1406,共5页Journal of Shanghai Jiao tong University:Medical Science

摘　　要：目的·探讨米非司酮在体外对人输卵管上皮纤毛摆动(CBF)及雌、孕激素受体表达的影响。方法·将25例人输卵管上皮组织在体外经米非司酮(0.1、1、10μmol/L)、孕酮10μmol/L、米非司酮(0.1、1、10μmol/L)+孕酮10μmol/L分别培养24 h后(另设空白对照组),测得CBF值;应用实时荧光定量PCR和免疫组织化学技术分析经米非司酮处理后人输卵管上雌激素受体α(ERα)和孕激素受体(PR)的变化;透射电子显微镜观察10μmol/L米非司酮对人输卵管上皮超微结构的影响。结果·与空白对照组相比,米非司酮在0.1、1、10μmol/L浓度时对输卵管CBF无显著影响(P=0.728,P=0.405,P=0.941);孕酮10μmol/L组CBF明显下降(P=0.000),米非司酮在0.1、1、10μmol/L浓度时,呈剂量-反应拮抗孕酮下调CBF的效应(P=0.484,P=0.000,P=0.000)。经米非司酮培养24 h后,输卵管上的ERα、PR表达上调;输卵管上皮纤毛超微结构与空白对照组比较,无明显差异。结论·米非司酮在人输卵管上发挥孕酮拮抗效应,这可能是这类常用口服紧急避孕药的输卵管避孕机制。Objective · To investigate ciliary beat frequency （CBF）, and estrogen and progesterone receptor expression levels of human fallopian tubes after mifepristone treating in vitro. Methods · Human fallopian tube mucosa explants （n=25） were treated with different concentrations of mifepristone （0.1, 1 and 10 μmol/L） or progesterone （10 μmol/L） separately, or both mifepristone and progesterone. After 24 h of treatment, CBF was measured. Quantitative real-time PCR and immunohistochemistry were used to research the expression of estrogen receptor-α （ERα） and progesterone receptor （PR） of human fallopian tubes after mifepristone treating. The ultrastructure of epithelial cells of fallopian tube after mifepristone （10 μmol/L） treating were observed with transmission electron microscopy. Results · The CBF at the concentrations of 0.1, 1 and 10 μmol/L was not affected by mifepristone （P=0.728, P=0.405 and P=0.941）. The CBF decreased markedly in the group of 10 μmol/L progesterone compared to control group （P=0.000）. Mifepristone （0.1, 1 and 10 μmol/L） dose dependently antagonized the progesterone-induced CBF decrease （P=0.484, P=0.000 and P=0.000）. Mifepristone upregulated the expression levels of ERα and PR in the fallopian tubes, but the ultrastructure of the cilia had no significant change. Conclusion · Mifepristone acts as progesterone antagonist in the human fallopian tube, which may explain the tubal contraceptive mechanism when used as an emergency contraceptive.

关 键 词：米非司酮 纤毛摆动频率 人输卵管 雌激素受体 孕激素受体 

分 类 号：R96[医药卫生—药理学]