Phanerochaete chrysosporium降解碳质金矿中元素碳高关联度变量的筛选  

Screening of High Correlation Variables of Elemental Carbon Degradation by Phanerochaete chrysosporium in Carbonaceous Gold Ores

作　　者：刘倩[1] 杨洪英 佟琳琳 

机构地区：[1]上海第二工业大学上海电子废弃物资源化协同创新中心,上海201209 [2]东北大学冶金学院,辽宁沈阳110819

出　　处：《黄金科学技术》2017年第5期140-144,共5页Gold Science and Technology

基　　金：国家自然科学基金项目"含砷复杂金矿细菌氧化矿物界面能及其作用机制的基础研究"(编号:U1608254);"难浸含砷金矿细菌氧化过程中[AsS]2-→As(III)→As(V)转化机理研究"(编号:51374066);辽宁省自然科学基金项目"利用ASH-07浸矿菌从含铜浮选尾矿中高效绿色回收铜的新技术研究"(编号:2014020037);上海第二工业大学2017校基金项目"黄孢原毛平革菌对废弃手机线路板中金属回收及作用机理研究"(编号:A01GY17EX19)联合资助

摘　　要：采用Plackett-Burman设计法对Phanerochaete chrysosporium降解碳质金矿中元素碳的高关联度变量进行筛选。通过前期的单因素试验确定影响真菌降解碳质物的8个变量,分别为愈创木酚浓度、糊精浓度、吐温-80浓度、草酸浓度、过氧化氢浓度、矿浆浓度、真菌浓度和作用时间。通过两水平的Plackett-Burman设计法确定影响真菌降解元素碳的高关联度变量,分别为愈创木酚浓度、草酸浓度和矿浆浓度。愈创木酚是一种诱导剂。低浓度时,愈创木酚通过提高酶活性和诱导一些酶产生,促进元素碳的降解。高浓度时,酶产生和酶活性受抑制,不利于元素碳的降解。草酸通过调节降解体系的p H值影响菌体生长及酶活性。矿浆浓度会影响元素碳和真菌的有效接触面积以及降解体系的剪切力和传质效率。The high correlation variables of the elemental carbon degradation with Rchrysosporium were selected in carbonaceous gold ores by Plackett-Burman design method.Eight variables of fungal degradation of carbonaceous matter were confirmed by the previous single factor experiments,which were the guaiacol concentration, the dextrin concentration, the tween-80 concentration, the oxalic acid concentration, the hydrogen peroxide concentration,the pulp density,the fungal concentration and the reaction time.The high correlation variables influencing the fungal degradation of elemental carbon, as identified by a two-level Plackett-Burman design method, were the guaiacol concentration, the oxalic acid concentration and the pulp density.Guaiacol is a kind of inducer.The low concentration of guaiacol could promote the degradation of elemental carbon by improving the enzyme activity and inducing the production of enzymes.The high concentration of gnaiacol was not conducive to the fungal degradation of elemental carbon because the generation of enzymes and the enzyme activity were inhibited.Oxalic acid could affect the fungal growth and the enzymes activity by adjusting pH value of the degradation system.The pulp density could influence the effective contact area between elemental carbon and fungi, the shear stress and the mass transfer efficiency of degradation system.

关 键 词：P.chrysosporium 元素碳 高关联度变量 Plackett-Burman设计法 筛选 碳质金矿 

分 类 号：TD953[矿业工程—选矿]