基于磁球表面的抗原决定基印迹法用于选择性识别细胞色素c的研究  

Epitope Imprinted Polymer on the Surface of Magnetic Nanoparticles for Selective Recognition of Cytochrome c

作　　者：赵晓丽[1] 何锡文[1] 李文友[1] 

机构地区：[1]药物化学生物学国家重点实验室天津市生物传感与分子识别重点实验室南开大学化学学院,天津300071

出　　处：《分析科学学报》2017年第5期619-625,共7页Journal of Analytical Science

基　　金：国家自然科学基金(No.21275078)

摘　　要：本工作合成了一种核壳型的抗原决定基磁性分子印迹聚合物,并用于选择性识别目标蛋白细胞色素c(Cytochrome c,Cyt c)。制备过程中先用溶剂热法合成Fe_3O_4磁性纳米粒子,然后加入Cyt c其C端的九肽作为模板,进行一段时间的预组装,最后加入多巴胺盐酸盐(DA)溶液,调节反应体系的p H使多巴胺聚合在磁球表面。洗脱掉模板后,即得到分子印迹聚合物。优化DA的用量使聚合物达到最佳的吸附效果。在最优条件下,制得的印迹聚合物对目标蛋白有较好的吸附选择性,并且有良好的重复利用性。此外,用抗原决定基做模板制得的聚合物的吸附容量和印迹因子明显优于用相应蛋白质做模板的情况。In this study, we synthesized a kind of core-shell magnetic molecular imprinting polymers （MIPs） with epitope imprinting method for the recognition of target protein cytochrome c（Cyt c）. Fe3O4 nanoparticles were synthesized through a solvothermal reaction firstly, then the epitope of Cyt c was added to preassemble for 2 h. After that, dopamine was added to form a thin polydopamine coating under alkaline conditions. The epitope imprinted polymers were obtained after washing with eluent. The polymers could reach a better adsorption effect after optimizing the amount of dopamine. The imprinted polymers exhibited better adsorption selectivity towards the target protein under the optimal conditions. Besides, the polymers showed reusability over continuous adsorption and elution processes. Moreover, the MlPs using Cyt c epitope as the template had better adsorption ability than those using Cyt c as the template.

关 键 词：抗原决定基印迹法 磁性纳米粒子 细胞色素C 

分 类 号：O657[理学—分析化学]