适配体功能化多壁碳纳米管整体柱分离富集细胞色素c  

Aptamer-modified Multi-walled Carbon Nanotubes Incorporated Monolithic Column for the Separation of Cytochrome c in Biological Samples

作　　者：周婵媛[1] 杜苑琪 杜卓[1] 李攻科[1] 肖小华[1] 

机构地区：[1]中山大学化学学院,广东广州510275

出　　处：《分析科学学报》2017年第5期631-636,共6页Journal of Analytical Science

基　　金：国家自然科学基金(No.21375155;21475153;21675178和21675179);广东省自然科学基金重点项目(No.2015A030311020);广东省公益研究与能力建设专项(No.2015A030401036);广州市科技计划(No.201604020165)

摘　　要：本文结合核酸适配体的特异性吸附及碳纳米管比表面积大的特点,研制了核酸适配体功能化碳纳米管/聚合物复合整体柱(AMMC),并将其应用于细胞色素c(Cyt c)选择性分离。结果表明,AMMC具有良好的通透性和稳定性,可选择性富集Cyt c,富集量可达1.47 mg。结合顺序注射系统,AMMC成功用于猪心样品中Cyt c的选择性分离富集,在生物样品分析中具有良好的应用前景。Efficient and sensitive separation methods are very important for biological study. In the present work, on the base of aptamer and multi-walled carbon nanotubes, an incorporated monolithic column（AMMC） was prepared and applied for the selective enrichment of cytochrome c. The AMMC exhibited good permeability,stability and selectivity,and a good adsorption capacity of 1.47 mg/mL for cytochrome c. Combined with sequential injection, cytochrome c in human plasma and pork heart was selectively obtained, showing good potential on the application of biological separation.

关 键 词：核酸适配体 碳纳米管 整体柱 细胞色素C 分离富集 

分 类 号：O658[理学—分析化学]